MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.Metapneumovirus was discovered in 2001 and is distributed worldwide. Infection in the upper respiratory tract is associated with mild illness, while lower respiratory tract infection can cause fever, cough, pneumonia, bronchiolitis and wheezing. Severe lower respiratory tract infection is most common in the very young, the very old and the immunosuppressed. Metapneumovirus is a common cause of respiratory infection.Phylogenetic analysis has shown two major genotypes, A and B, each containing two sub-divisions, suggesting that four distinct lineages circulate in the population with different lineages predominating in different years. M3884-49 recognizes the nucleoprotein of all sub-types (A1, A2, B1 and B2) of hMPV and may be used in conjunction with M3884-49A, M3884-49B, and M3884-49C to detect all strains of hMPV.M3884-49 does not cross-react with cell cultures infected with hRSV, influenzaviruses A and B, adenovirus, parainfluenza viruses 1, 2, 3 and 4b, mumps virus, measles virus, varicella-zoster virus, herpes simplex virus types 1 and 2, human cytomegalovirus, human Herpes virus type 6, ECHOvirus 19, Coxsachievirus B4, poliovirus types 1-3, HHV6 or uninfected HeLa, MA104, 3MK, LLC-MK, HEp2, MRC-5 and HSB-2 cells. It also does not react with sputum bacteria.ApplicationsSuitable for use in Immunofluorescence. Other applications not tested.Recommended DilutionsOptimal dilutions to be determined by the researcher.Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.Note: Applications are based on unconjugated antibody.ImmunogenSub­type A hMPV virus isolate NCL03­4/145.FormSupplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.PurityPurified by Protein A affinity chromatography from tissue culture supernatant.SpecificityRecognizes the nucleoprotein of all sub-types (A1, A2, B1 and B2) of human metapneumovirus (hMPV).References1. Fenwick, F. et al. (2007) Diagnosis of human metapneumovirus by immunofluorescence staining with monoclonal antibodies in the North­East of England. J. Clin. Virol. 40: 193­196

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