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Usbio/W1018-40D-ML550 Rabbit Anti-West Nile Virus, Core, CT (WNV Core, Capsid Protein C) (MaxLight 550)/W1018-40D-ML550/100ul
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MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.WNV belongs to the Flaviviridae family which includes closely viruses such as Dengue fever, Yellow fever and Tick borne encephalitis. West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway. West Nile Virus is believed to have originated in the West Nile region of Africa and has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health.ApplicationsSuitable for use in FLISA, Immunofluorescence and Western Blot. Other applications not tested. Recommended DilutionsFLISA: 1ug/mlImmunofluorescence: 20ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher. Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Note: Applications are based on unconjugated antibody.ImmunogenA peptide corresponding to a 15aa sequence from near the C-terminus of West Nile Virus core protein.FormSupplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.PurityPurified by affinity chromatography.SpecificityRecognizes the 14kD core protein of West Nile Virus (WNV).References1. Gould, L.H., et al (2004) West Nile virus: a growing concern. J.Clin. Invest. 113:1102-1107 2. Chu, J.J., et al (2005) Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein. J. Gen. Virol. 86:405-12 3. Chu, J.J., et al (2004) Interaction of West Nile virus with a v ß 3 integrin mediates virus entry into cells. J. Biol. Chem. 2004; 279:54533-41. 4. Yang, J.S., et al (2002) Induction of inflammation by West Nile Virus capsid through the caspase-9 apoptotic pathway. Emerg. Infect. Dis. 8:1379-84.

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