MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.Applications: |Suitable for use in Immunofluorescence, Flow Cytometry, Immunoblot, Methylated DNA Immunoprecipitation, and Immunohistochemistry (frozen and paraffin). Other applications not tested.Recommended DilutionFlow Cytometry: 10ul labels 10e6 cells in 100ulImmunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.Optimal dilutions to be determined by the researcher.Hybridoma: |Sp2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice.Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Note: Applications are based on unconjugated antibody.Immunogen5-Methylcytidine.FormSupplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.PurityPurifiedSpecificityRecognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. Species Crossreactivity: human, rat, mouse.References1. Reynaud, C. et al. (1992) Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies. Cancer Lett. 61: 255-262. 2. Habib, M. et al. (1999) DNA Global Hypomethylation in EBV - Transformed Interphase Nuclei. Exp. Cell. Res. 249: 46-53. 3. Hernandez-Blazquez, F. et al. (2000) Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gut. 47: 689-693. 4. Giraldo, A. M. et al. (2007) DNA methylation and histone acetylation patterns in cultured bovine fibroblasts for nuclear transfer. Mol. Reprod. Dev. 74: 1514-1524.

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