Pseudomonas aeruginosa is a Gram-negative bacterium widely distributed in nature and causing opportunistic infections in humans. P. aeruginosa is an important bacterial pathogen of nosocomial (hospital derived) infections, and it can also cause life threatening diseases in patients with cancer, burn wounds, cystic fibrosis and those that have received immuno- suppressive therapy. Classification of P. aeruginosa isolates is an important and routine task in hospitals. The basis for the serotyping system for P. aeruginosa is the differences among the O antigen side chains of lipopolysaccharide (LPS). A large number of O antigen side chains have been established by various investigators around the world which led to the creation of a standardized serotyping system known as the International Antigenic Typing Scheme (IATS) with twenty characterized O serotype strains of P. aeruginosa.Applications: |Suitable for use in ELISA, Agglutination assays (10 assays). Other applications not tested.Recommended Dilution: |Optimal dilutions to be determined by the researcher.Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Immunogen:|FormSupplied as a lyophilized powder. Reconstitute with 115ul ddH2O.PurityAscitesSpecificityRecognizes O12 serotype of Pseudomonas aeruginosa.References1. Lam, J.S., Anderson, E.M., and Hao, Y. (2014). LPS quantitation procedures. Methods Mol Biol 1149, 375-402. 2. Lam, J.S., MacDonald, L.A., Lam, M.Y.C., Duchesne, L.G.M., and Southam, G.G. (1987). Production and characterization of monoclonal antibodies against serotype strains of Pseudomonas aeruginosa. Infect Immun 55, 1051-1057.

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