首页 > Usbio > Usbio/172094-AF-AF555 Mouse Anti-Fibronectin (Cold Insoluble Globulin, CIG, FINC, FN1, FNZ, GFND, GFND2, LETS, Migration Stimulating Factor, MSF, Ugl-Y3) (BSA & Azide Free) (AlexaFluor®555)/172094-AF-AF555/100ul

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Usbio/172094-AF-AF555 Mouse Anti-Fibronectin (Cold Insoluble Globulin, CIG, FINC, FN1, FNZ, GFND, GFND2, LETS, Migration Stimulating Factor, MSF, Ugl-Y3) (BSA & Azide Free) (AlexaFluor®555)/172094-AF-AF555/100ul

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Fibronectinisadimericglycoproteinof440kD,whichispresentincells,extracellularmatrix,andblood.Itpossessesatleastfourbindingsitesforcollagen,glycosaminoglycans,transglutaminase,andacellsurfacereceptor.Fibronectinisinvolvedincelladhesion,tissueorganization,andwoundhealing.ThisMAbisdirectedagainstthepeptidecoreandreactswithboththeplasmaandcellularformsoffibronectin.Itblocksthefibronectin-medicatedcellattachmentnotbydisruptingthecollagen-fibronectininteraction,butbyinterferingwiththeattachmentoffibronectintoitsreceptoronthecellsurface.Applications:|SuitableforuseinImmunofluorescence,FlowCytometry,ELISA,WesternBlot,Immunoprecipitation,Immunohistochemistry.Otherapplicationsnottested.RecommendedDilution:|FlowCytometry:0.5-1ug/millioncells|Immunofluorescence:0.5-1ug/ml|WesternBlot:0.5-1ug/ml|Immunoprecipitation:0.5-1µg/500ugproteinlysate|Immunohistochemistry(Frozen&Formalin-fixed):0.5-1ug/mlfor30minutesatRT|(Stainingofformalin-fixedtissuesrequiresboilingtissuesectionsin10mMcitratebuffer,pH6.0,for10-20minfollowedbycoolingatRTfor20minutes)|Optimaldilutionforaspecificapplicationshouldbedetermined.PositiveControlSW156cellsorKidneyStorageandStABIlityMaybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ImmunogenHumanfibronectinpurifiedfromserumbyaffinitychromatographyongelatin-sepharose.CellularLocalization:ConnectivetissuematrixFormSuppliedasaliquidinPBS,pH7.2.PurityPurifiedbyProteinA/Gaffinitychromatography.SpecificityRecognizeshumanFibronectin.References1.SchoenRC,et.al.Hybridoma,1982,1(2):99-108.

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