首页 > Usbio > Usbio/172036-AF-AF555 Mouse Anti-Bromodeoxyuridine (Bromodeoxyuridine, BUdr, BrdU) (BSA & Azide Free) (AlexaFluor®555)/172036-AF-AF555/100ul

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Usbio/172036-AF-AF555 Mouse Anti-Bromodeoxyuridine (Bromodeoxyuridine, BUdr, BrdU) (BSA & Azide Free) (AlexaFluor®555)/172036-AF-AF555/100ul

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172036-AF-AF555

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产品说明

ItreactswithBromodeoxyuridine(BrdU)insinglestrandedDNA(producedbypartialdenaturationofdoublestrandedDNA),BrdUcoupledtoaproteincarrier,aswellasfreeBrdU.BrdUisathymidineanalog,incorporatedintocellnucleiduringDNAsynthesispriortomitosis.AntibodytoBrdUishelpfulindetectingS-phasecells,providingusefulinformationontheaggressivenessoftumors.Applications:|SuitableforuseinImmunofluorescence,FlowCytometry,Immunohistochemistry.Otherapplicationsnottested.RecommendedDilutionFlowCytometry:0.5-1ug/millioncellsImmunoprecipitation:0.5-1ug/millioncellsImmunofluorescence:0.5-1ug/mlImmunohistochemistry(Frozen&Formalin-fixed):0.5-1ug/mlfor30minutesatRT(UseAbat1ug/mlfor30minuteatRT[Forstainingofformalin-fixedtissues,incubatesectionsin4NHClfor30minutesatRTfollowedbydigestionwithtrypsinat1mg/mlPBS,10minat37oC(Cat.#AP-9008)]Optimaldilutionforaspecificapplicationshouldbedetermined.PositiveControlCellsgrowninpresenceofBrdUortissuesfromexperimentalanimalsinjectedwithBrdUStorageandStABIlityMaybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ImmunogenBromodeoxyuridine(BrdU)conjugatedtoKLH.CellularLocalization:NuclearFormSuppliedasaliquidinPBS,pH7.2.PurityPurifiedbyProteinA/Gaffinitychromatography.SpecificityRecognizesBromodeoxyuridine.SpeciesCrossreactivity:allspecies.References1.WelbergJWet.al.JournalofClinicalPathology,1990,43(6):453-6.2.WilliamsLSet.al.Cytometry,1990,11(4):490-7.

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