MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.Applications: |Suitable for use in RIA, Western Blot and Immunohistochemistry. Other applications not tested.Recommended DilutionImmunohistochemistry: Frozen and formalin fixed tissues.Optimal dilutions to be determined by the researcher.Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Note: Applications are based on unconjugated antibody.ImmunogenMyosin purified from myofibrils isolated from histochemically mixed human skeletal muscle.FormSupplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.PurityPurified IgGSpecificityRecognizes the human slow myosin heavy chain. Clearly identifies Type 1 fibers. Within skeletal muscle is specific for slow myosin heavy chain in a wide variety of species. It reacts strongly with rat and feline slow myosin heavy chain. Identifies beta (slow) myosin heavy chain in heart ventricles.References1. Journal of Cell Biology 104:447–459 (1987). 2. J. Neurological Sciences 81:19–43 (1987). 3. J. Neurological Sciences 83:93–108 (1988). 4. J. Muscle Res. Cell Motility 9:30–47 (1988). 5. ‘Immunochemistry’ (chapter 6) In: Muscle Biopsy. A Practical Approach, 2nd edition, London: Bailliere Tindall (1985)

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