MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (400nm); Emission (423nm); Extinction Coefficient 32,000.RSV is so named from the formation of syncytia, or multinucleated masses of fused cells. Two virally specified surface glycoproteins are prominent in RSV. The large glycoprotein is designated G and the other fusion protein is designated F. In addition, two distinct groups may be present in RSV, Group A and/or Group B.Applications: |Suitable for use in Indirect Immunofluorescence, FLISA, Immunohistochemistry and Fusion inhibition. Other applications not tested.Recommended DilutionsIndirect Immunofluorescenc: 1:480FLISA: 1:10,000Fusion inhibition: >1:256Immunohistochemistry: Paraffin sectionsOptimal dilutions to be determined by the researcher.Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.ImmunogenHuman RSV isolateFormSupplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.PurityPurified by ammonium sulfate precipitation.SpecificityRecognizes all RSV antigens. Does not react with Para 1-3, Influenza A & B and Adenovirus by IFA. Non reactive with HEp-2 cells and WI-38 cells.References1. Schepens B, et al. (2011) The Journal of infectious diseases.204:1692-701. Epub 2011 Oct 12. 2. Differential pathogenesis of respiratory syncytial virus clinical isolates in BALBc mice. (2011) Journal of virology.85:5782-93 3. Hinzey A, et al. (2011) Endocrinology.152:483-94. Epub 2010 Dec 29. 4. Hinzey A, et al. (2011) Endocrinology.152:483-94. Epub 2010 Dec 29. 5. Swedan S, et al. (2009) Journal of virology.83:9682-9693 6. Toby M Ward, et al. (2008) The Journal of general virology.89:741-50 7. Weidong Zhang, et al. (2004) Journal of virology.78:13717-26 8. Bitko, Vira, et al. (2003) BMC Microbiol., 3: 9 (2003) 9. K K Tekkanat, et al. (2001) Journal of immunology (Baltimore, Md. : 1950).166:3542-8 10. H Matsuse, et al. (2000) Journal of immunology (Baltimore, Md. : 1950).164:6583-92 11. M Kumar, et al. (1999) Vaccine.18:558-67. 12. Mohapatra, SS. et al.US Patent Application, 00095: 13. Matsuse, H et al. (2000) J Immuno, 164:6583-6592 (2000) 14. Miller, AL et al. (2003) J Immuno, 170:3348-3356 (2003) 15.Tekkanat, KK et al. (2001) J Immuno, 166:3542-3548 (2001)

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