MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.ERR alpha is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site specific transcription regulator and has also been shown to interact with estrogen and the transcription factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium chain acyl coenzyme A dehydrogenase (MCAD),and thyroid hormone receptor genes. ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in brain, spinal cord, pituitary gland and heart. More recently, ERR alpha has been observed in various human breast cancer cell lines, breast adipose tissue and breast tumors. Studies demonstrating the use of ERR alpha antagonists in treating breast cancer have excited great interest in this receptor in the search for curing the disease (Chisamore, 2009).Applications: |Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended DilutionWestern Blot: 2ug/mlImmunocytochemistry: 1:500Optimal dilutions to be determined by the researcher.Positive ControlHeLa cell lysateStorage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Note: Applications are based on unconjugated antibody.ImmunogenLinear peptide corresponding to human ERR-alpha (KLH).FormSupplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.PurityPurified by Protein A affinity chromatography.SpecificityRecognizes human ERR-alpha P2. Species Crossreactivity: mouse

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