MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.||ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. Research has demonstrated the existence of at least two isoforms of ATG12.||Applications: |Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Western Blot: 1ug/ml|Immunohistochemistry (Paraffin): 2.5ug/ml|Optimal dilutions to be determined by the researcher.||IHC Positive Control Tissue: |Human brain||Recommended Secondary Antibodies:|I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.Product Type:Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide corresponding to a 15aa sequence from near the center of human ATG12.|Specificity: Recognizes human Autophagy-related protein 12 (ATG12). Detects a band of approximately 40 kDa in mouse heart tissue lysate.Species Crossreactivity: mouse, rat|||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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