MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.||In mammalian cells, Autophagy-related protein 7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Studies indicate that caspase-8 may alter ATG7 levels and thus the ATG7 program of autophagic cell death. ATG7 is evolutionally conserved and exhibits high homology within mammalian species. It expressed in wide range of tissues including kidney, liver, bone marrow and lymph nodes.||Applications: |Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Western Blot: 1:10-1:50|Immunohistochemistry (Frozen): 1:10-1:50|Optimal dilutions to be determined by the researcher.||Recommended Secondary Antibodies:|I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb|I1904-40J: IgG, Fc (HRP) Pab Gt xRb|I1904-40P: IgG, Fc, X-Adsorbed (HRP) Pab Gt xRb||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.Product Type:Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™750.|Purity: Purified by immunoaffinity chromatography.|Immunogen: A peptide corresponding to an 17 amino acid sequence from near the carboxy terminus of human ATG7.|Specificity: Recognizes human Autophagy-related protein 7, a 78kD cytoplasmic protein. Species Crossreactivity: mouse|||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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