AFP is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adults. |Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down’s syndrome. ||Applications: |Suitable for use in ELISA. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Hybridoma:|Immunized Balb/c mice were fused with cells of the Sp2/0 myeloma cell line. ||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.Product Type:Mab|Isotype: IgG2a|Clone No: 4A3|Host: mouse|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified|Immunogen: Purified human alpha fetoprotein.|Specificity: Recognizes alpha fetoprotein (AFP), a member of the albuminoid superfamily. Does not cross-react with human albumin. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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