The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependent manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30nm chromatin fiber. ||Applications:|Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. ||Recommended Dilutions:|Immunofluorescence: Speckled staining is observed in the nucleus of normal and malignant cells.|Western Blot: 1:100. In blots of rat brain homogenate a duplet is observed at 27-29kD consistent with Histone H1. |Immunohistochemistry (Frozen, paraffin): Acetone or PFA fixation of frozen sections is recommended.|Optimal dilutions to be determined by the researcher. ||Immunohistochemistry Positive Control:|Tonsil||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Further dilutions can be made in assay buffer.||Note: Applications are based on unconjugated antibody.Product Type:Mab|Isotype: IgG2a|Clone No: AE-4|Host: mouse|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Nuclei from myeloid leukamia biopsy cells.|Specificity: Recognizes human Histone H1. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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