B. burgdorferi differ in their susceptibility to normal human serum and are therefore classified as complement-resistant, complement-sensitive and intermediate complement-sensitive. Complement resistant bacteria absorb human immune regulators FHL-1/reconectin and factor H using two outer surface Complement Regulator-Acquiring Surface Proteins (CRASP-1 and CRASP-2). Surface-attached FHL-1/reconectin retains its complement regulatory and cleavage activity (Kraiczy, et al., 2001). Factor H and FHL-1 serve as cofactors for factor I, a serine protease that cleaves complement component 3b (C3b) directly on the cell surface and thereby ensures resistance of spirochetes to complement-mediated lysis (Kraiczya, et al., 2007). It is possible that because of discontinuous binding regions in the factor H/FHL-1, a long-distance interaction may be involved in binding of both immune regulators. Putative coiled-coil structural elements may also be important in the interaction of B. burgdorferi CRASP-1 with factor H (Kraiczya, et al., 2007). These proteins represent another immune evasion mechanism of B. burgdorferi, as bacteria acquire human complement regulators to control complement activation on their surface and prevent formation of toxic activation products(Kraiczy, et al., 2001).||Applications: |Suitable for use in ELISA, Western Blot. Other applications not tested.||Recommended Dilution:|ELISA: 1:1000|Western Blot: 1:1000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Product Type:Pab|Isotype: IgG|Host: Rabbit|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Recombinant protein corresponding to B. burgdorferi CRASP-2, fused to MBP-Tag. |Specificity: Recognizes Borrelia burgdorferi CRASP-2, strain B31 (ATCC 35210), derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). BLAST analysis suggest reactivity with CRASP-2 from B. burgdorferi sources based on 100% homology with the immunizing sequence. Partial|cross-reactivity is expected against B. garinii, B. spielmanii, and valaisiana|sources based on 91-89% homology. Cross-reactivity with CRASP-2 from|other sources has not been determined.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。

恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！