The severe acute respiratory syndrome coronavirus (SARS-CoV) is the virus that causes the severe acute respiratory syndrome (SARS) which is a serious, potentially life-threatening viral infection. The spike (S) protein of coronaviruses is responsible for receptor binding and membrane fusion. It is a type I integral membrane protein, which shares similarity with class I virus fusion proteins, and is N-glycosylated and trimerized in the endoplasmic reticulum. The S protein contains the determinants for host specificity, cell tropism, and pathogenesis. The S protein is also able to stimulate humoral and cellular immune responses, and therefore, it is one of the major targets for the development of vaccine candidates.||Applications: |Suitable for use in Flow Cytometry, ELISA, Western Blot, Neutralization. Other applications not tested.||Recommended Dilution:|Flow Cytometry: 1:20|Western Blot: 1:2000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Product Type:Mab|Isotype: IgG1|Clone No: Spike|Host: mouse|Concentration: Not determined |Form: Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.|Purity: Ascites|Specificity: Recognizes Sd10 fragment of SARS-CoV Spike Protein.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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