ADAMTS2, A disintegrin and metalloproteinase with thrombospondin motifs 2, also known as procollagen I N-proteinase (PC I-NP), is an enzyme that in humans is encoded by the ADAMTS2 gene. The ADAMTS2 gene contains 22 exons. The human ADAMTS2 gene is mapped to chromosome 5q23-q24 by analysis of somatic cell hybrids. ADAMTS2 is responsible for processing several types of procollagen proteins. Procollagens are the precursors of collagens, the proteins that add strength and support to many body tissues. Specifically, this enzyme clips a short chain of amino acids off one end of the procollagen. This clipping step is necessary for collagen molecules to function normally and assemble into fibrils outside cells||Applications:|Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Western Blot: 0.1-0.5ug/ml|Immunohistochemistry (Fozen/paraffin): 0.5-1ug/ml. Antigen retrieval by boiling paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is required for the staining of paraffin sections.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||Note: Applications are based on unconjugated antibody.Product Type:Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Snthetic peptide corresponding to aa1189-1204, KTRNQRIQELIDEMRK from the C-terminal of human ADAMTS2. Species Sequence Homology: mouse|Specificity: Recognizes humanADAMTS2. No crossreactivity with other proteins.Species Crossreactivity: rat||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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