MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.||Lysosomes are membrane-bound organelles found within the cytoplasm of most cells, which contain hydrolytic enzymes and act as the major compartment for heterophagic and autophagic digestion. Members of the lysosomal-associated membrane protein family (LAMPS) are believed to play an important role in protecting the lysosomal membrane from protease degradation and are involved in lectin-mediated cell adhesion. Evidence shows that CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway and human studies have revealed a correlation between the level of surface expression of CD107b on tumor cells and their metastatic potential.||Applications: |Suitable for use in Western Blot, Electron Microscopy, Immunoprecipitation, Immunofluorescence and Flow Cytometry. Other applications not tested.||Recommended Dilutions:|Western Blot: 1:100-1:500|Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required for this application. |Optimal dilutions to be determined by the researcher.||Hybridoma:|Spleen cells from immunized BALB/c mice were fused with cells of the NS1 myeloma cell line. ||Recommended Negative Control:|I1904-78R: IgG1 Murine Negative Control||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.Product Type:Mab|Isotype: IgG1|Clone No: 9A182 (AC17)|Host: mouse|Source: canine|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: MDCK (Madin-Darby Canine Kidney) cells.|Specificity: Recognizes canine CD107b, a 95kD heavily-glycosylated type I integral lysosomal glycoprotein. Demonstrates staining pattern consistant with localization to lysosome.|Species Crossreactivity: human and mink. Does not react with mouse or rat||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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