MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (400nm); Emission (423nm); Extinction Coefficient 32,000.||Surfactant protein D (pSP-D), is a 138kD member of the collectin family of proteins. Surfactant protein D is thought to play a significant role in the innate immune response against microorganisms within the lungs and at other mucosal surfaces. SP-D is a multifunctional protein which binds selectively to saccharides and lipids on microbial surfaces promoting antimicrobial responses and regulating surfactant lipid homeostasis. SP-D expression is induced in lung surfactant in response to aerogenous bacterial infection, and upon binding to bacteria, promotes phagocytosis and antigen presentation by dendritic cells. Recent studies suggest that pSP-D could serve as a serum biomarker specific for bronchopneumonia in pigs.||Applications: |Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Immunohistochemistry (paraffin): 1ug/ml, this product requires antigen retrieval using heat treatment prior to staining of paraffin sections.|Optimal dilutions to be determined by the researcher.||Hybridoma: |P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. ||Positive Control:|Porcine lung tissue||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.Product Type:Mab|Isotype: IgG1|Clone No: 1.7|Host: mouse|Source: porcine|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™405.|Purity: Purified by Protein G affinity chromatography.|Immunogen: Purified porcine surfactant protein D|Specificity: Recognizes porcine surfactant protein D (pSP-D).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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