Receptor Activator of Nuclear Factor kappa B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is also expressed on dendritic cells and facilitates immune signaling. RANKL (Receptor Activator for Nuclear Factor kappa B Ligand) is found on the surface of stromal cells, osteoblasts, and T cells. By analysis of somatic cell and radiation hybrid panels, Anderson et al. (1997) mapped the RANK gene to 18q22.1.||Applications: |Suitable for use in ELISA and Western Blot. Other applications not tested.||Recommended Dilution:|ELISA: 0.1-0.5ug/ml|Western Blot: 0.1-0.5ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Product Type:Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~0.5mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Recombinant protein corresponding to mouse RANK expressed in E. coli. (Position: C35-S214). Species sequence homology: rat (96%)|Specificity: Recognizes mouse RANK. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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