The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.||Applications: |Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Western Blot: 0.1-0.5ug/ml|Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Product Type:Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: ~0.5mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg thimerosal, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to 0.5mg/ml.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide corresponding to aa203-218, ATVHLEQGGTKLVKDL, from human Sonic Hedgehog at N-terminus, identical to the related rat and mouse sequences.|Specificity: Recognizes human Sonic Hedgehog. Species Crossreactivity: mouse and rat||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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