Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.||Applications: |Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.||Recommended Dilution:|ELISA: 1:10,000|Western Blot: 1:500-1:2000|Immunohistochemistry: 1:200-1:1000|Immunofluorescence (IF-IC): 1:100-1:500|ELISA (peptide): 1:20,000-1:40,000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Product Type:Mab|Isotype: IgG1|Host: Mouse|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.|Purity: Purified by affinity chromatography.|Immunogen: Purified recombinant fragment corresponding to human SYP expressed in E. coli.|Specificity: Recognizes endogenous levels of total human SYP. Species Crossreactivity: Rat||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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