产品说明
F4/80 is a 160kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: ≤1ug/10e6 cells in 100ul volume or 100ul of whole blood.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Product Type:Mab|Isotype: IgG2b,k|Clone No: CI:A3-1|Host: Rat|Source: mouse|Concentration: ~0.5mg/ml|Form: Supplied as a liquid in PBS, 0.05% Proclin300, 1% BSA. Labeled with Biotin.|Purity: Purified|Specificity: Recognizes mouse F4/80.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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