Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.||Applications: |Suitable for use in Immunofluorescence,Western Blot, Immunoprecipitation, Immunohistochemistry, CHIP. Other applications not tested.||Recommended Dilution:|Immunofluorescence: 1:200-1:500|Western Blot: 1:1000-1:3000|Immunohistochemistry: 1:200-1:500|Immunoprecipitation: 1:20-1:50|CHIP: 1:20-1:50|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Product Type:Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: ~1mg/ml |Form: Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic acetylated peptide corresponding to residues surrounding K14 of human Histone H3.|Specificity: Recognizes endogenous levels of human Histone H3 protein only when acetylated at K14. Species Crossreactivity: mouse, rat, Other (Wide Range)||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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