产品说明
Myosin is a highly conserved, ubiquitously expressed protein that interacts with actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of nonphosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain (MLC) by myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.||Applications: |Suitable for use in Western Blot. Other applications not tested.||Recommended Dilution: |Western Blot: 1:500-1:1000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Product Type:Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: ~1mg/ml |Form: Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide corresponding to human MYH6.|Specificity: Recognizes endogenous levels of human MYH6/7 protein. Species Crossreactivity: mouse and rat||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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