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OverviewTheIV.3antibodyreactswithhumanCD32(FcγRII),an~40kDatype1transmembraneglycoproteinthatmediatesseveralfunctionsincludingphagocytosis,cytotoxicity,immunomodulationandplateletaggregation.CD32isencodedbythreegenes(A,B,C)andatleast6isoformsaregeneratedviaalternativemRNAsplicing,i.e.,IIa1,IIa2,IIb1,IIb2,IIb3andIIc.Allisoformsareexpressedbymonocytes/macrophages,placentaltrophoblastsandendothelialcells.Inaddition,theIIbisoformisexpressedbyBcells,andtheIIaisoformbyplatelets,granulocytesand,weakly,byBcells.IsoformIIcisexpressedbyNKcellsandneutrophils.CD32bindsweaklytotheFcregionofmonomericIgGbutmorestronglytoIgGaggregatesandimmunecomplexes.Theseinteractionscanresultinnon-specificlabelinginantibody-baseddetectionandcellseparationexperimentsandtheIV.3antibodymaybeemployedasablockingantibodytoreducenon-specificbinding.TheIV.3antibodybindsmoststronglytotheIIaisoformsofCD32,withtheepitopemappedtoaminoacids132-137[FSHLDP]indomain2,withintheligandbindingsite.BindingoftheIV.3antibodycanbeblockedbycloneFLI8.26inflowcytometryanalyses,suggestingthattheseclonesmayshareacommonoroverlappingepitope.ThisantibodyclonehasbeenverifiedforpurityassessmentsofcellsisolatedwithEasySep™kits,includingEasySep™HumanTCellEnrichmentKit(Catalog#19051)andEasySep™HumanCD4+TCellEnrichmentKit(Catalog#19052).Subtype:PrimaryAntibodiesTargetAntigen:CD32AlternativeNames:FCRII,FcγRIIReactiveSpecies:HumanConjugation:FITC;UnconjugatedHostSpecies:MouseCellType:BCells;GranulocytesandSubsets;MonocytesApplication:CellIsolation;FlowCytometry;FunctionalAssay;Immunocytochemistry;Immunohistochemistry;NeutralizationandBlocking;WesternBlottingAreaofInterest:ImmunologyClone:IV.3GeneID:2212Isotype:IgG2b,kappa
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