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Stemcell/BrainPhys™ Neuronal Medium/05793

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OverviewBrainPhys™ Neuronal Medium is a serum-free neuronal basal medium. BrainPhys™ may be used to culture primary neurons or neurons derived from human pluripotent stem cells (hPSCs). Based on the formulation published by Cedric Bardy and Fred H. Gage (C Bardy et al. PNAS, 2015), BrainPhys™ is more representative of the central nervous system extracellular environment and increases the proportion of synaptically active neurons.Applications of BrainPhys™ Neuronal Medium include culture of primary neurons, differentiation and maturation of hPSC-derived neurons, microelectrode array-based recording of neuronal activity, live fluorescent imaging (including calcium imaging and optogenetics) and transdifferentiation of somatic cells to neurons.To ensure cell survival in long-term serum-free culture, BrainPhys™ must be combined with an appropriate supplement. For your convenience, various BrainPhys™ kits are available for primary or hPSC-derived neurons. BrainPhys™ Neuronal Medium and SM1 Kit (Catalog #05792) is recommended for culture of primary neurons. BrainPhys™ Primary Neuron Kit (Catalog #05794) is recommended for plating and culture of primary neurons. BrainPhys™ Neuronal Medium N2-A & SM1 Kit (Catalog #05793) and BrainPhys™ hPSC Neuron Kit (Catalog #05795) are recommended for the differentiation and maturation of hPSC-derived neurons, in combination with lineage-specific growth factors and/or small molecules (if necessary).Advantages:• More representative of the brain’s extracellular environment• Improved neuronal function and a higher proportion of synaptically active neurons• Perform functional assays without changing media and shocking cells• Supports long-term culture of ES/iPS cell- and CNS-derived neurons• Rigorous raw material screening and quality control ensure minimal lot-to-lot variabilityComponents: BrainPhys™ Neuronal Medium and SM1 Kit (Catalog #05792) BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790)NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711) BrainPhys™ Neuronal Medium N2-A & SM1 Kit (Catalog #05793) BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790)NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711)N2 Supplement-A, 5 mL (Catalog #07152) BrainPhys™ Primary Neuron Kit (Catalog #05794) NeuroCult™ Neuronal Plating Medium, 100 mL (Catalog #05713)NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711)BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790) BrainPhys™ hPSC Neuron Kit (Catalog #05795) BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790)NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711)N2 Supplement-A, 5 mL (Catalog #07152)Human Recombinant BDNF, 10 µg (Catalog #78005)Human Recombinant GDNF, 10 µg (Catalog #78058) Subtype:Basal Media; Specialized MediaCell Type:Neural Cells, PSC-Derived; Neurons; Pluripotent Stem CellsSpecies:Human; Mouse; RatApplication:Cell Culture; Differentiation; MaintenanceBrand:BrainPhysArea of Interest:Disease Modeling; Drug Discovery and Toxicity Testing; Neuroscience; Stem Cell BiologyFormulation:Serum-Free

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