Species: Human Host Species: E. coli MW: see Description Genbank #: NM_001228 Tag(s): C-terminal His-tag a.a: 200 – 496 (end) Background:Caspase-8 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Caspase-8 has been associated with chronic inflammation and neurodegenerative disorders including Alzheimer’s, Huntington’s, and Parkinson’s. Description:Human Caspase 8 (CASP8), GenBank Accession No. NM_001228), a.a. 200 - 496 (end), with C-terminal His-tag, MW=34.5 kDa, expressed in an E. coli cell expression system. Procaspase-8 is autocleaved between the prodomain and large protease subunit (p18). The cleavage product, p30, contains both the large (p18) and small (p10 [His-tag]) protease subunits. p30 is further processed to the p18 and p10 subunits, which dimerize to form active enzyme. UniProt Q14790 Synonym(s): Caspase-8, CASP8, Caspase 8, FLICE, MACH, Mch5 Specific Activity: ≥ 1145 pmol/min/µg Assay Conditions: Assayed using BPSs Homogeneous Caspase-8 Assay Kit, Cat. # 80704. 50 mM HEPES, pH 7.2,50 mM NaCl, 10 mM EDTA, 0.1% Chaps,5% glycerol, 10 mM DTT, and 20 µMCaspase 8 substrate. Incubate for 30minutes at room temperature. Fluorescenceintensity is measured at exc380/em505 nm. Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT and 20% glycerol. Format: Aqueous buffer solution Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling. Reference(s): 1. MacPherson, G., et al., J. Natl.Cancer Inst. 2004 Dec 15;96(24):1866-9.2. Sun, T., et al., Nat Genet. 2007 May;39(5):605-13.3. Hoffman, J.C., et al. Mol. Cell. Biol. 2009 Aug; 29(16): 4431-4440. Warning(s): Avoid freeze/thaw cycles. Scientific Category: Protease Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.