Species: Human Host Species: E. coli MW: 52.5kDa Genbank #: NM_152615, var2 Tag(s): N-terminal GST-tag a.a: 221–444(end) Description:Human poly (ADP-ribose) polymerase family, member 15 (PARP15) (GenBank Accession No. NM_152615, var.2), a.a. 221-444(end) with N-terminal GST-tag, MW = 52.5 kDa, expressed in an E. coli expression system. UniProt Q460N3 Synonym(s): poly (ADP-ribose) polymerase 15, poly(ADP-ribosyl)transferase 15, PARP15 Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. Assay Conditions: Enzyme reaction is conducted usingBPSs PARP15 Assay Kit (Catalog #80567) at room temperature for 30 minutes. Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol. Format: Aqueous buffer solution Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s):

1. Andersson CD., et al. J. Med. Chem. 2012 Sep 13;55(17):7706-18.2. Ricardo C. T. Aguiar., et al. J. Biochem.2005 Oct 280;(40):33756–65.

Warning(s): Avoid freeze/thaw cycles. Scientific Category: PARP Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.