Species: Human Host Species: Sf9 insect cells MW: 86 kDa (complex) Genbank #: NM_006892(DNMT3B), NM_013369 (DNMT3L) Tag(s): N-terminal His, N-terminal GST-tag a.a: 564–853(end), DNMT3b; 160–387(end), DNMT3LDescription:Complex of human DNA methyl-transferase 3B (DNMT3B), GenBank Accession No. NM_006892, a.a. 564-853(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system. UniProt Q9UBC3 (DNMT3B), Q9UJW3 (DNMT3L)Specific Activity: ≥0.52 pmol/min/µg. Assay Conditions: Assayed using BPS Bioscience DNMT3B Assay Kit, Cat. #52034. Add 50 µl reaction mixcontaining DNMT3B/DNMT3L to wells coatedwith DNMT substrate. Incubate 2 hr at 37°C.Add antibody against 5-methylcytosine, thenHRP-labeled secondary antibody followed bychemiluminescent detection. Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, and 20% glycerolFormat: Aqueous buffer solutionStorage / Stability:

>6 months at –80°C.

Application(s): Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.Warning(s): Avoid freeze/thaw cycles.Scientific Category: MethyltransferaseProduct Type: Recombinant ProteinData shown is lot-specific. Contact us for specific information on other lots.