Species: Human Host Species: Sf9 insect cells MW: 92 kDa Genbank #: NM_033116 Tag(s): N-terminal GST-tag a.a: 1–979 (end), delta 346–731 Description:Human NEK9, also known as NERCC (GenBank Accession No. NM_033116), a.a 1-345, 732-end (a.a. 1-979 with deleted fragment a.a. 346-731), with N-terminal GST-tag, MW =92 kDa, expressed in Sf9 insect cells via a baculovirus expression system. UniProt Q8TD19 Synonym(s): NEK9, NIMA-related kinase 9, Never In Mitosis-Related Kinase 9, NERCC Specific Activity: 147 pmol/min/µg Assay Conditions: Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml MBP for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega). Formulation: 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT. Format: Aqueous buffer solution Storage / Stability:

>6 months at -80°C.

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Roig, J., et al. Genes Dev. 16: 1640-1658 (2002).2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001). Warning(s): Avoid freeze/thaw cycles Scientific Category: Kinase/Serine-Threonine Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.