Species: Human Host Species: Sf9 Insect Cells MW: CDK1: 60kDa; CyclinB1: 75kDa Genbank #: CDK1: NM_001786; CyclinB1: NM_031966 Tag(s): N-terminal GST-tag a.a: Full-length Description:Human CDK1/CyclinB1complex, also known as CDC and CCNB1,CCNB respectively. GenBank AccessionNos. NM_001786 and NM_031966respectively, full-length with N-terminal GST-tagson both proteins, expressed in abaculovirus-infected Sf9 cell expressionsystem. MW = 60 kDa (CDK1) MW = 75 kDa(CyclinB1). UniProt CDK1: P06493; CyclinB1: P14635 Synonym(s): Cyclin-dependent kinase 1, CDC and CCNB1, CCNB respectively Specific Activity: 90 pmol/min/μgAssay Conditions: Assay was done in aKinase buffer using Histone H1 (0.1 mg/ml)as a substrate with 20
M ATP at 30°C for 45min. The amount of ATP transferred wascalculated using Kinase-Glo reagent(Promega). Formulation: 40 mM Tris pH 8.0, 110 mMNaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mMDTT and 20% glycerol. Format: Aqueous buffer solution Storage / Stability: At least 6 months at -80°C Application(s): Useful for studying enzyme kinetics and screening inhibitors. Reference(s):

1. Vantieghem, A. et al., J. Biol. Chem.2002; 277(40): 37718-37731.

2. Rigas, A.C. et al., Oncogene. 2007;26(55): 7611-7619.

Warning(s): Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 – 0.5%). Scientific Category: Kinase-Serine/Threonine Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.