MW: 652.7 Da Formula: C₃₁H₄₅FN₄O₁₀ Target: Caspase 6 Background:VEID is the specific recognition sequence for caspase-6/Mch2. Caspase 6 is only one of at least three effector caspases, the others being caspase 3 and 7, that are active during caspasedependent apoptosisLoss of DNA-binding activity and TNFα-induced apoptosis can be prevented by Z-VEID-FMK. The caspase-6-speci?c inhibitor Z-VEID-FMK signi?cantly prevented apoptosis of caspase-6-microinjected neurons, and was also able to abolish the cleavage of procaspase-8, although caspase-6 is activated downstream of caspase-8 in Fas-mediated apoptosis. Description:Z-VEID-FMK is a synthetic peptide that irreversibly inhibits activity of VEID-dependent caspases (e.g., caspase-6). The inhibitor is designed as a methyl ester to facilitate cell permeability. Can be used for both in vitro and in vivo studies--see note for using this product in vitro. Synonym(s): Caspase-6 inhibitor (fluoromethylketone); Benzyloxycarbonyl-Val-Glu(OMe)-Ile-?Asp(OMe)-fluoromethylketone Purity:

≥90% by HPLC

Biological Activity: We recommend using 2-10 µM for inhibiting caspase activity in cell culture. The optimal dose may vary for different cells and culture conditions. Solubility: Soluble in DMSO (20 mM) Storage / Stability:

Store at or below –20°C. Solid form is stable at least 12 months from date of receipt, when stored as directed. Do not store aqueous solutions for more than one day.

Notes: The inhibitor is designed as a methyl ester to facilitate cell permeability. If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups. Amino Acid Sequence: Z-Val-Glu(OMe)-Ile-Asp(OMe)-CH?F Scientific Category: Apoptosis Inhibitor Product Type: Inhibitor Data shown is lot-specific. Contact us for specific information on other lots.