Species: Human Host Species: E. coli MW: 60.4 kDa Genbank #: NM_004566 Tag(s): N-terminal His-tag a.a: 2–520(end) Description:Human 6-phosphofructo-2-kinase/ fructose-2,6-biphosphatase 3, also known as PFKFB3 and IPFK2, (GenBank Accession No. NM_004566), a.a. 2-520(end)) with N-terminal His-tag, MW = 60.4 kDa, expressed in an E. coli cell expression system. UniProt Q16875 Synonym(s): 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3, PFKFB3, PFK2, IPFK2 Specific Activity: ≥28.5 pmole/min/µg. Assay Conditions:

The reaction was done in 50 mM Tris-HCl, pH 7.5, 12.5 mM MgCl2, 1 mM β-D-fructose-6-phosphate, 100 μM ATP, 2 mM DTT, 5 mM KH2PO4, and various amount of PFKFB3 in a final volume of 50 μL. The reaction was incubated at 30°C for 30 min, and the amount of ATP consumed was calculated using Kinase-Glo^® Plus reagent (Promega).

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 360 mM imidazole and 20% glycerol Format: Aqueous buffer solution Storage / Stability:

>6 months at –80°C

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Cavalier, M.C., et al., Proteins 2012;80(4):1143-1153.2. Reddy, M.M., et al., Leukemia 2012;26(3):481-489. Warning(s): Avoid freeze/thaw cycles. Scientific Category: Metabolic Enzymes-Kinase Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.