首页 > AAT Bioquest > AAT Bioquest/trFluor™ Eu goat anti-rabbit IgG (H+L) *Cross Adsorbed*/16847/1 mg

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AAT Bioquest/trFluor™ Eu goat anti-rabbit IgG (H+L) *Cross Adsorbed*/16847/1 mg

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OverviewPrinterFriendlyVersionEx/Em(nm)346/617MWN/ACAS#N/ASolventWaterStorageF/D/LCategoryImmunologyFluorescentAnti-IgGsRelatediFluor™DyesandKitsTagAntibodiesBiochemicalAssaysManyBIOLOGicalcompoundspresentincells,serumorotherbiologicalfluidsarenaturallyfluorescent,andthustheuseofconventional,promptfluorophoresleadstoseriouslimitationsinassaysensitivityduetothehighbackgroundcausedbytheautofluorescenceofthebiologicalmoleculestobeassayed.Theuseoflong-livedfluorophorescombinedwithtime-resolveddetection(adelaybetweenexcitationandemissiondetection)minimizespromptfluorescenceinterferences.OurtrFluor™Euprobesenabletime-resolvedfluorometry(TRF)fortheassaysthatrequirehighsensitivity.trFluor™EuprobeshavelargeStokesshiftsandextremelylongemissionhalf-liveswhencomparedtomoretrADItionalfluorophoressuchasAlexaFluororcyaninedyes.ComparedtotheotherTRFcompounds,ourtrFluor™EuprobeshaverelativelyhighstABIlity,highemissionyieldandabilitytobelinkedtobiomolecules.ThistrFluor™Eugoatanti-rabbitIgG(H+L)conjugateiscommonlyusedasasecondstepreagentforindirectimmunofluorescentstaining,whenusedinconjunctionwithprimaryantibodies.SpectrumAdvancedSpectrumViewer #xAxis div{ display: inline-block; position: relative; left: 0; margin: 0px 35.5px; padding: 5px; } #graphContainer{ display: inline-block; white-space: nowrap; margin: 0 auto; } #visIBLeSpectrum{display:block;margin-left:50px;padding-right:150px;background-color:#000;height:10px;width:653px;}#yAxis{display:inline-block;height:220px;width:50px;}#graphArea{display:inline-block;border-left:solid3pxblack;border-bottom:solid2pxblack;position:relative;height:220px;width:650px}#graphGrid{position:absolute;top:0;left:0;height:220px;width:650px;overflow:hidden;}#graphGridpath{shape-rendering:crispEdges;}#graphContent{position:absolute;top:0;left:0;height:220px;width:650px;}#hoverText{white-space:normal;background-color:#EFEFEF;display:inline-block;width:150px;height:222px;border-left:solid1px#AFAFAF;border-bottom:solid2pxblack;border-right:solid1px#AFAFAF;vertical-align:top;font-size:14px;text-align:center;padding:5px15px;box-sizing:border-box;line-height:150%;}.noselect{-webkit-touch-callout:none;/*iOSSafari*/-webkit-user-select:none;/*Safari*/-khtml-user-select:none;/*KonquerorHTML*/-moz-user-select:none;/*Firefox*/-ms-user-select:none;/*InternetExplorer/Edge*/user-select:none;/*Non-prefixedversion,currentlysupportedbyChromeandOpera*/}Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG. #grid{ stroke: #DFDFDF; stroke-width: 1; } Sorry,yourbrowserdoesnotsupportinlineSVG. .graphLine{ fill-opacity:0.5; stroke-opacity:0.75; } .graphLine:hover{ fill-opacity:0.75; stroke-opacity:1; } Sorry,yourbrowserdoesnotsupportinlineSVG.Movemouseovergridtodisplaywavelength&intensityvalues.300400500600700800900Wavelength(nm)References&CitationsPrinterFriendlyVersion1. SavilleL,SpaisC,MasonJL,AlbomMS,MurthyS,MeyerSL,AtorMA,AngelesTS,HustenJ.(2012)Time-ResolvedFluorescenceResonanceEnergyTransferasaVersatileToolintheDevelopmentofHomogeneousCellularKinaseAssays.AssayDrugDevTechnol.2. LoMC,NgoR,DaiK,LiC,LiangL,LeeJ,EmkeyR,EksterowiczJ,VenturaM,YoungSW,XiaoSH.(2012)Developmentofatime-resolvedfluorescenceresonanceenergytransferassayforcyclin-dependentkinase4andidentificationofitsATP-noncompetitiveinhibitors.AnalBiochem,421,368.3. PailaYD,KombrabailM,KrishnamoorthyG,ChattopadhyayA.(2011)OligomerizationoftheSEROtonin(1A)receptorinlivecells:atime-resolvedfluorescenceanisotropyapproach.JPhysChemB,115,11439.4. MartikkalaE,Rozwandowicz-JansenA,HanninenP,Petaja-RepoU,HarmaH.(2011)Ahomogeneoussingle-labeltime-resolvedfluorescencecAMPassay.JBiomolScreen,16,356.5. GaboritN,LarbouretC,VallagheJ,PeyrussonF,Bascoul-MolleviC,CrapezE,AzriaD,ChardesT,PoulMA,MathisG,BazinH,PelegrinA.(2011)Time-resolvedfluorescenceresonanceenergytransfer(TR-FRET)toanalyzethedisruptionofEGFR/HER2dimers:anewmethodtoevaluatetheefficiencyoftargetedtherapyusingmonoclonalantibodies.JBiolChem,286,11337.6. LeyrisJP,RouxT,TrinquetE,VerdieP,FehrentzJA,OueslatiN,DouzonS,BourrierE,LamarqueL,GagneD,GalleyrandJC,M"KadmiC,MartinezJ,MaryS,BaneresJL,MarieJ.(2011)Homogeneoustime-resolvedfluorescence-basedassaytoscreenforligandstargetingthegrowthhormonesecretagoguereceptortype1a.AnalBiochem,408,253.7. PosokhovYO,KyrychenkoA,LadokhinAS.(2010)Steady-stateandtime-resolvedfluorescencequenchingwithtransitionmetalionsasshort-distanceprobesforproteinconformation.AnalBiochem,407,284.8. Alvarez-CurtoE,WardRJ,PedianiJD,MilliganG.(2010)LigandregulationofthequaternaryorganizationofcellsurfaceM3muscarinicacetylcholinereceptorsanalyzedbyfluorescenceresonanceenergytransfer(FRET)imagingandhomogeneoustime-resolvedFRET.JBiolChem,285,23318.9. KotaS,ScampaviaL,SpicerT,BeelerAB,TakahashiV,SnyderJK,PorcoJA,HodderP,StrosbergAD.(2010)Atime-resolvedfluorescence-resonanceenergytransferassayforidentifyinginhibitorsofhepatitisCviruscoredimerization.AssayDrugDevTechnol,8,96.10. VisserAJ,LaptenokSP,VisserNV,vanHoekA,BirchDJ,BrochonJC,BorstJW.(2010)Time-resolvedFRETfluorescencespectroscopyofvisiblefluorescentproteinpairs.EurBiophysJ,39,241.ViewAllAsPDF

美国AAT Bioquest Inc.(前身是ABD Bioquest，Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域，包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品，快速地丰富各个领域的产品。