DESCRIPTION:Afullyvalidated,highlyspecificaffinitypurifiedrabbitpolyclonalantibodytoαandβisoformsofthreonine-286phosphorylatedCaMKIICa2+/calmodulin-dependentkinaseII(CaMKII)isaubiquitous,multifunctionalserine/threoninekinaseinvolvedintranslatingCa2+-transientsignalsintocellularresponses.FourseparateCaMKIIgenesareexpressedinman(α,β,δ,γ)withisoformswithineachgene.CaMKIIδandγareabundantintheheart.CaMKIIisactivatedbyCa2+/CaMbinding,andbecomesactivatedforprolongedperiodsoftimefollowingautophosphorylationofThr-286orThr-287(equivalentresiduesindifferentisoforms).Thisantibodyrecognizestheactive,autophosphorylatedenzyme(allisoforms)andthuscanmeasuretheabundanceofactiveCaMKII.ProlongedactivationofCaMKIIisassociatedwithavarietyofvascularandcardiacdiseasesincludingstroke,atrialfibrillationcardiacarrhythmia&suddendeath,cardiacapoptosis,andheartfailure.Antibodyform:LyophilisedProteinAAffinityPurifiedAntibody(IgG). Speciesrecognition:rat,humanandmouse.Expectedtoreactwithvariousisoformsfromchicken,cow,pig,rabbit,orangutan,zebrafish,ferretandXenopuslaevis. DATASHEET:DescriptionAffinitypurifiedrabbitpolyclonalantibodytoCaMKIIphosphothreonine-286/287ImmunogenSyntheticpeptide(M281HRQET(PO3H2)VDC289)correspondingtoaminoacidssurroundingthephosphorylatedthreonineresidueatposition286or287ofCaMKII,whichwasconjugatedtokeyholelimpethemocyanin(KLH)bycarbodiimidecrosslinkage.IsotypeIgGPurificationProteinAaffinitypurifiedSpeciesreactivityReactswithrat.Notyettestedinotherspecies.However,fromprimarysequenceanalysis,theantibodywillrecognisePhosphoThr-286(or287)inallisoforms(α,β,γandδ)fromhuman,ratandmouse.Theantibodyisalsoexpectedtoreactwithvariousisoformsfromchicken,cow,pig,rabbit,orangutan,zebrafish,ferretandXenopuslaevis.TestedapplicationsWBRecommendeddilutionWB1:1000OptimizationOptimaldilutionstobedeterminedbyenduserStorageLyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin50µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles.StorageLyophilised:4°C.Reconstituted:-80°CDatasheetA010-50APMSDSA010-50APRegulatorystatementProductforresearchuseonly.Notintendedfordiagnosticortherapeuticuse.
Badrilla一氧化氮(NO)在调节心血管功能中起重要作用。除了经典的cGMP依赖途径的NO激活外,NO还可以通过蛋白S-亚硝基化来调节细胞功能,S-亚硝基化是氧化还原依赖性,基于硫醇的可逆翻译后蛋白质修饰,涉及将NO部分连接至亲核蛋白巯基组。
对调节蛋白S-亚硝基化的机制的更好理解将为我们提供新的治疗见识和干预心血管疾病的靶标。Badrilla提供越来越多的抗体和检测试剂盒,以支持您的一氧化氮和S-亚硝酰基化研究项目;这些产品中的每一个的更多详细信息可以在下面找到。
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Name Product Code
$ 368.00
产品编号: A010-105
方便易用的试剂盒,结合了“生物素转换”方法的改进,可以直接在整个细胞或组织中可视化S-亚硝基化蛋白质,并通过蛋白质印迹分析。
添加到购物篮
改良的“生物素开关”测定
标记样品中的所有S-亚硝基化位点(“组学”)
检测全细胞,组织或裂解物中的S-NO蛋白
色度或荧光检测
足够的试剂用于3 x 20样品
$ 341.30
产品编号: A010-2E11
亲和纯化的小鼠单克隆抗体对酪氨酸的克隆2E11
添加到购物篮
$ 341.30
产品代码: A010-6B2AP
亲和纯化的小鼠单克隆抗体对硝基酪氨酸的克隆6B2(NY)
添加到购物篮
$ 441.00
产品编号: A010-513
方便的试剂盒,其中包含高完整性的硝基酪氨酸单克隆抗体以及相关的阳性和阴性对照。