Product detailsImagesReferencesIsotypeMouse IgG2bSpecificityThe antibody MEM-85 reacts with an extracellular antigen of both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.WorkshopHLDA IV: WS Code NL 706; HLDA VI: WS Code AS Ref.15ApplicationFC (QC tested)Application detailsFlow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.ReactivityHumanImmunogenLeukocytes of a patient suffering from LGL Type Leukaemia.Other namesPGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHRPreparationThe purified antibody is conjugated with tandem dye APC-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.FormulationStabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azideStorage and handlingStore in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Exbio licence noteThe product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden.Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.Licence noteCy™ and CyDye™ are registered trademarks of Cytiva. Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD44 (MEM-85) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood). Separation of human lymphocytes (red-filled) from CD44 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD44 (MEM-85) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood). General references:Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2007 Dec 12 PubMed Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA. CD44 regulates macrophage recruitment to the lung in lipopolysaccharide-induced airway disease. Am J Respir Cell Mol Biol. 2007 Aug;37(2):248-53. PubMed Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G. Down-regulation of CD44 contributes to the differentiation of HL-60 cells induced by ATRA or HMBA. Cell Mol Immunol. 2007 Feb;4(1):59-63. PubMed Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp Mol Pathol. 2007 Dec;83(3):341-6. PubMed Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. Exp Mol Pathol. 2007 Oct;83(2):207-15. PubMed Product specific references:Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61.PubMed Schmidt D, Achermann J, Odermatt B, Breymann C, Mol A, Genoni M, Zund G, Hoerstrup SP: Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source. Circulation. 2007 Sep 11;116(11 Suppl):I64-70.PubMed Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15;109(6):2545-52.PubMed Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8.PubMed Bazil V, Strominger JL: Metalloprotease and serine protease are involved in cleavage of CD43, CD44, and CD16 from stimulated human granulocytes. Induction of cleavage of L-selectin via CD16. J Immunol. 1994 Feb 1;152(3):1314-22.PubMed Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Stefanová I, Hilgert I, Bazil V, Kristofová H, Horejsí V: Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules. Immunogenetics. 1989;29(6):402-4. Campioni D, Moretti S, Ferrari L, Punturieri M, Castoldi GL, Lanza F: Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment. Haematologica. 2006 Mar;91(3):364-8.PubMed