首页 > IBA-lifesciences > IBA-lifesciences/CD45RA Fab Streptamer® Isolation Kit MB, human/for isolation out of 1x10^9 cells with magnetic microbeads (MB);to be used with a permanent magnet/6-8000-208

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IBA-lifesciences/CD45RA Fab Streptamer® Isolation Kit MB, human/for isolation out of 1x10^9 cells with magnetic microbeads (MB);to be used with a permanent magnet/6-8000-208

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6-8000-208

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IBA-lifesciences

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For isolation of CD45RA+ T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer® components for positive cell isolation with magnetic microbeads*and for removal of Streptamers from isolated cells.1.) Strep-Tactin® Magnetic Microbeads for Fab Streptamers; 2 vials for 5x10^8 cells each 2.) CD45RA Fab-Strep, human for isolation of T-cells; 2 vials for 5x10^8 cells each3.) D-Biotin stock solution for Streptamer® technology; 2x 1 ml4.) Buffer IS, Streptamer® Washing Buffer, 10x concentrated; 50 mlThis product requires dry ice shipment and is shipped directly from our Headquarters in Germany.*Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 ml and 2 x 50 ml Falcon tubes.Principle of Selection:Isolation of human CD45RA+ T cells is performed by positive selection. PBMCs are labeled with magnetic CD45RA Fab Streptamers®. Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet. This CD45RA positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position). The magnetic Streptamers migrate toward the tube wall and the label-free cells remain in the supernatant. Biotin is removed by washing. The resulting cell preparation is highly enriched with CD45RA+T cells with a purity of more than 90%. No depletion steps and no columns are needed.

德国IBA GmbH致力于向生命科学研究提供高质量的工具。作为一个 "TAGnology" 公司，IBA是一个合格的学术界研究伙伴，是表达克隆和蛋白质纯化技术以及蛋白质和核酸递送技术的最前沿的供应商之一，其专利的蛋白质产品和服务覆盖整个药物发现过程。IBAGmbH总部座落于德国的哥廷根，它从2000年开始在世界范围的市场营销其产品和服务。Strep-tag是一个通用的专利技术平台，用于克隆、表达、一步纯化、检测和分析纯的、具有生物学活性的蛋白质。在双标签蛋白质中，作为Strep-tag的最佳伙伴，IBA还提供6 xHistidine-tag 用以对全长表达进行快速控制，并且由于采用这两种独立的纯化方法而增加蛋白质纯度。新技术MATra ("Magnet Assisted Transfection") 可以有效转染大范围的细胞系。MALipofection Enhancer与新的脂质体转染试剂IBAfect的组合是一个绝佳的“磁力辅助的脂质体转染”组合。Streptamer技术是功能性T细胞分离的一个突破，它是一种对抗原特异T细胞进行功能性分离和鉴定的新方法。IBA最新开发的Twin-Strep-Tag系统用于离析多蛋白质复合体以及鉴定蛋白质-蛋白质相互作用。2007年底，IBA隆重推出了组合式克隆的新产品StarGate，用以系统地将任何目的基因转入大肠杆菌、酵母、哺乳动物等背景细胞，高速和方便地筛选优化的宿主/标签组合