DescriptionTheImmunoStarSEROtoninantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofrathypothalamus,raphenucleiandspinalcordusingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/20,000-1/40,000inPBS/0.3%TritonX-100-biotin/avidin-HRPTechnique.Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywith25ugofserotonin/BSA.CrossreactivityofSerotoninantiserawasexamined.With5µg,10µgand25µgamountsthefollowingsubstancesdidnotreactwithSerotoninantiseradiluted1/20,000usingtheBn-SA/HRPlabelingmethod:5-hydroxytryptophan,5-hydroxyindole-3-aceticacid,anddopamine.PhotoDescription:LowmagnificationIHCimageofneuronsstainingforthe5-HTrabbitantibodyintheraphenucleusoftheratbrainstem(above),andcorrespondinghighmagnificationimageoftheraphenucleus(below).Thetissuewasfixedwith4%formaldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:25000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.Host:RabbitQuantity/Volume:100µLState:LyophilizedWholeSerumAvailABIlity:InStockReactsWith:Abalone(HaliotisKamtschatkana),Amphibian,AnnelidMushroom,Annelida(SegmentedWorm),Ant,Ant(PheidoleDentata),AntalisEntalisScaphopoda,Aplysia(SeaSlug),AplysiaCalifornica(SeaSlug),AplysiaPunctata(SeaSlug),Arachnocampa(Gnat),ArchidorisPseudoargus(SeaSlug),AsterinaPectinifera(Starfish),AstyanaxMexicanus(CaveFish),Axolotl(Salamander),Bacteria,Beetle,Bird,Brachiopods,Bryozoans(Polyzoa),Buffalo,BufoMarinus(Toad),Bug,Bumblebee,Butterfly,Butterfly(Monarch),Canine,Cat,Chick,Chicken,Chiton,ChrysoperlaCarneaGreenLacewing,Clam,ClioneLimacinaPteropod(FruitBat),Cockroach,Cod,Crab,Crayfish,Cricket,Crustaceans,CupienniusSalei(Spider),DesertLocust,Dog,Dogfish(Shark),Dove,Drosophila(Fly),DrosophilaMelanogaster(Fly),featherStar,Ferret,Fish,Fish(Salmon),Flounder,Fly,Frog,Frog(XenopusLaevis),FruitFly,Gastropod(Slug),Gastropod(Slug),Gerbil,Glowworm,Goldfish,Grasshopper,GuineaPig,Hamster,Hedgehog,HelisomaTrivolvis(Snail),Hen,HermitCrab,Honeybee,Human,IdiosepiusNotoides(Squid),IlyanassaObsoleta(SeaSnail),Insect,Kitten(Cat),Lamprey,Leech,Lizard,Lobster,Lobster(Cycliophora),LobsterLarva,Locust,LythrypnusDalli(Fish),Macracantha(Cephalocarida),ManducaSexta(Moth),Marmoset(Monkey),Mercenaria(Clam),Mexicansalamander(Ambystoma Mexicanum),Mollusca,Mollusk,Monkey,Monkey(Macaque),Mosquito,Mosquito(AedesAegypti),Moth,Moth(ManducaSexta),Mouse,Mudpuppy,Mussel,Newt,Nudibanch,NudibranchMollusc,Nudipleura,Octopus,Onychophora(VelvetWorm),Ovine(Sheep),ParhedyleCryptophthalma(SeaSlug),PhoronisPallida(Phoronida),PhyllodoceMaculata(Phyllodocidae),PhyllodoceMaculata(Polychaeta),Pig,Pigeon,PilidiumLarvae,PlatynereisDumerilii(Ragworm),Platypus,PleurobranchaeaCalifornica(SeaSlug),PleurobranchaeaJaponica(SeaSlug),Pleurodeles(Newt),Prawn,PrayingMantis,PtychoderaFlava(Worm),Quail,Rabbit,RajaErinacea(Skate),Ram(Sheep),RanaPerezi(Frog),Rat,RedAbalone,RedAbalone,RenillaKoellikeri(Cnidarian),RhodniusProlixus(Bug),RhodniusProlixus(Parasite),Salamander,SandDollar,SchistocercaGregaria(Locust),ScorpaenaPorcus(Fish),Scorpion,ScyliorhinusCanicula(Dogshark),SeaBass(Fish),SchistocercaGregaria(Locust),ScorpaenaPorcus(Fish),Scorpion,ScyliorhinusCanicula(Dogshark),SeaBass(Fish),SeaCucumber,SeaLamprey,SeaLily,SeaScallop,SeaSlug,SeaSpider,SeaStar,SeaUrchin,Seahorse,Shark,Sheep,Shrew,Skate,Snail,SparusAurata(Fish),Spisula(Clam),Springtails,SquirrelMonkey,StingRay,StomatopodCrustacean,Sturgon,Tapeworm,TecturaScutum(Mollusk),Tick,TigriopusCalifornicus(Copepod),Toad,TragulusJavanicus(MouseDeer),TritoniaDiomedea(SeaSlug),Trout,Turkey,Turtle,Worm,Worm(Digenea),Worm(DiphyllobothriumDentriticum),Worm(DugesiaTigrina),Worm(DugesiaTigrina),Worm(PhyllodoceGroenlandica),Worm(PolychaeteCapitella),ZebraFinch(Bird),ZebrafishAlternateNames: anti-5-HTRabbitDRRI: AB_572263Immunogen:SerotoninGeneSymbol:SplEntrezGeneID:104153