ELISpotPLUS for enumeration of cells secreting TNF-αThis kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting porcine TNF-α. The kit includes ELISpot plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate.Pre-coated plates reduce assay time and minimize variability.Porcine TNF-α ELISpotPLUS kits are also available with Streptavidin-HRP and TMB substrate. See Related products.Analyte InformationTNF-αTumor necrosis factor-α (TNF-α, also known as TNF-alpha, TNF-a, TNFa, and Tumor necrosis factor-alpha) is produced by many different cell types e.g. monocytes, macrophages, T cells and B cells. Among the many effects of TNF-α, are protection against bacterial infection, cell growth modulation, immune system regulation and involvement in septic shock.Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.Email:Send your order toorder@mabtech.com.Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com. Application:ELISpotReactivity:Pig Documentation:Datasheet/ProtocolSDS Contents:Biotinylated detection mAb (MT859)Streptavidin-ALPSubstrate (BCIP/NBT-plus)2 Pre-coated white plates (mAb MT32E9)Size:2 platesOther formats:Click to view all Pig TNF-α products I have compared Mabtech ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples. Mabtech"s kits proved to be superior to our protocol, had significantly lower background and provided better and higher detection of spots.