产品说明
BasicELISpotforenumerationofIgGsecretingBcellsHumanIgGELISpotBASICenablesenumerationofBcellssecretingtotalIgGaswellasantigen-specificIgG.Itcontainsamatchedpairofmonoclonalcaptureanddetectionantibodies,Streptavidin-HRPandpolyclonalactivators.Antigen-specificBcellscanbeenumeratedintwoalternativeways;bycoatingwithantigenorbyusingbiotinylatedantigeninthedetectionstep.FormemoryBcells,werecommendpre-stimulationwithR848andrecombinanthumanIL-2(bothincluded).IgGELISpotBASICisalsoavailablewithStreptavidin-ALP.SeeRelatedproducts.Ascomplementaryproducts,weofferELISpotplatesandsubstrates.SeeRelatedproducts.Speciescross-reactivityThemonoclonalantibodiescross-reactwithIgGfromnon-humanprimates.R848andhumanIL-2canbeusedforstimulationofBcellsfromnon-humanprimatesSeetheHuman/NHPcross-reactivitytable.AnalyteInformationIgGIgG(ImmunoglobulinG)isthemostabundantIgisotypeinserumandmakesupapproximately80%ofallserumImmunoglobulins.Inhumans,therearefoursubclassesofIgGwithhighestserumconcentrationsofIgG1followedbyIgG2,IgG3andIgG4.InmicetheIgGsubclassesaredefinedIgG1,IgG2a/c,IgG2bandIgG3.TheIgGmoleculeconsistsoftwoheavyandtwolightschains(κorλ)resultinginamoleculewithtwoarmsforantigenbinding.HighlevelsofIgGantibodiesareinducedfollowingtheinitialIgMresponseinatypicalimmuneresponsetoantigens.Application:ELISpotReactivity:Human/NHPContents:CapturemAbs(MT91/145)BiotinylateddetectionmAbs(MT78/145)Streptavidin-HRPR848RecombinanthumanIL-2Size:for4platesOtherformats:ClicktoviewallHuman/NHPIgGproductsIhavecomparedMabtechELISpotassaytoELISpotassayswerunatourcompanyonregularbasis"head-to-head"usingidenticalsamples.Mabtech"skitsprovedtobesuperiortoourprotocol,hadsignificantlylowerbackgroundandprovidedbetterandhigherdetectionofspots.
Mabtech的主要检测目标包括:
Amyloid-β、IgG2、apoA1、IgG2a、apoB、IgG2b、apoD、IgG2c、apoE、IgG3、apoH、IgG4、apoJ (Clusterin)、IgM、apoM、IL-1β、CD3、IL-2、CD4、IL-4、CD8、IL-5、CD28、IL-6、CD40、IL-8 (CXCL8)、CD43、IL-10、Galectin-3、IL-12 (p70)、GM-CSF、IL-12/-23 (p40)、Granzyme A、IL-13、Granzyme B、IL-17A、IFN-α2、IL-21、IFN-αpan、IL-22、IFN-γ、IL-23、Ig kappa、IL-29 (IFN-λ1)、Ig lambda、IL-31、IgA、Insulin、IgA1、Perforin、IgE、TGF-β1 (Latent TGF-β1)、IgEa、hioredoxin-1、IgG、TNF-α、IgG1
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