首页 > GenWay > GenWay/ACTH Ultra Sensitive lumELISA (Mouse/Rat)*/GWB-16A89F/ELISA_Kits

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GenWay/ACTH Ultra Sensitive lumELISA (Mouse/Rat)*/GWB-16A89F/ELISA_Kits

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GWB-16A89F

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GenWay

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4000-520-616

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Description:IntendedUseTheGenWayInc.ACTHChemiluninescenceELISA(lumELISA)isanultrasensitivemethod(Lessthan1pg/mL)intendedforthequantitativedeterminationofACTH(AdrenocorticotropicHormone)inMouse/Ratplasma.SummaryandExplanationAdrenocorticotropicHormone(ACTH)isa39-aminoacidpeptidehormone(MW=4500)secretedmainlybytheanteriorpituitarygland.Varioustypesofstressorpainperceivedinhigherlevelsofthebrainmodulatesecretionofthehypothalamicneurosecretoryhormone,corticotropinreleasinghormone(CRH).CRHstimulatespituitaryACTHsecretion.ThesecondpeptidethatmodulatesACTHsecretionisvasopressin(AVP).AVPsecretionisalsostimulatedbystressandactssynergisticallywithCRHtoincreaseACTHsecretioninthepituitaryportalcirculation.PrincipleoftheTestTheGenWayIncACTHImmunoassayisatwo-sitelumELISA(ChemiluninescenceEnzyme-LinkedImmunoSorbentAssay)forthemeasurementoftheBIOLOGicallyactive39aminoacidchainofACTH.AgoatpolyclonalantibodytoACTH,purifiedbyaffinitychromatography,andamousemonoclonalantibodytoACTHarespecificforwell-definedregionsontheACTHmolecule.OneantibodyispreparedtobindonlytheC-terminalACTH34-39andthisantibodyisbiotinylated.Theotherantibodyispreparedtobindonlythemid-regionandN-terminalACTH1-24andthisantibodyislabeledwithhorserADIshperoxidase[HRP]fordetection.Inthisassay,calibrators,controls,orsamplesaresimultaneouslyincubatedwiththeenzymelabeledantibodyandabiotincoupledantibodyinastreptavidin-coatedmicroplatewell.Attheendoftheassayincubation,themicrowelliswashedtoremoveunboundcomponents.Upontheadditionoftheluminolsubstrate,theenzymeactivityintheenzyme-boundfractionisdirectlyproportionaltotheconcentrationoftheACTHinthesample.Astandardcurveispreparedrelatinglightunit(RLU)totheconcentrationoftheACTH.ConcentrationsofACTHpresentinthecontrolsandsamplesaredetermineddirectlyfromthiscurve.SensitivityThesensitivityofthisassayisdefinedasthesmallestsinglevalue,whichcanbedistinguishedfromzeroatthe95%confidencelimit.TheCBIACTHlumELISAhasacalculatedsensitivityofLessthan1pg/mL.AdditionalInformation:NameACTHUltraSensitivelumELISA(Mouse/Rat)*RelatedProductNamesACTHUltraSensitivelumELISA(Mouse/Rat)ELISAKitMolecularWeight0.5IntendedUseResearchUseOnly

GenWay生物科技是一家领先的蛋白质和抗体解决方案供应商。自1998年以来，他们已经提供蛋白质的解决方案和应用研究。迄今为止，GenWay生物科技的在圣地亚哥工厂产生了数以千计的蛋白质和抗体。公司致力于为客户提供研究界最好的试剂。如果产品不符合既定的规格，公司将全额退款。