首页 > GenWay > GenWay/Rat Fibronectin ELISA Kit/GWB-16BC00/96 well

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GenWay/Rat Fibronectin ELISA Kit/GWB-16BC00/96 well

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GWB-16BC00

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AdditionalInformation:NameRatFibronectinELISAKitRelatedProductNamesRatFibronectinELISAKitAliasSymbolsFibronectin1,FN1,FNProteinNameFibronectinDescriptionofTargetFibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InvolvedinosteoblastcompacSwissprotIDP04937SpeciesReactivityRatAssayInfoAssayMethodology:QuantitativeSandwichELISASensitivity2.697ng/mlKitRange6.25ng/ml-200ng/mlHomologyRatStandardRangeReferToManualSampleTypeSerum,plasmaandotherBIOLOGicalfluidsProductFormatInSolutionSpecimen/VolumesReferToManualApplicationsELISAReconstitutionandStorage1.DILUENTThe5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.2.WASHSOLUTIONThe20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.3.ENZYME-ANTIBODYCONJUGATEUndilutedhorserADIshperoxidaseanti-FCTconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.4.CHROMOGEN-SUBSTRATESOLUTIONTheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.5.STOPSOLUTIONTheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.6.ANTI-RATFIBRONECTINELISAMICROPLATEAnti-RatFCTcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.7.RATFIBRONECTINCALIBRATORThelyophilizedRatFibronectincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.ReactivityRatKitSize96wellKitPrincipleGenWayBiotechFibronectinELISAKit(Rat)(GWB-16BC00)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforFibronectinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Fibronectin-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleFibronectincapturedinthewell.KitComponentComponentAmountAnti-RatFibronectinMicroplate96Wells(12x8WellStrips)20XDiluentConcentrate50mL20XWashSolution50mL100XEnzyme-AntibodyConjugate150uLChromogen-SubstrateSolution12mLStopSolution12mLRatFibronectinCalibrator1KitDuration100MinutesStorageRefertokitinstructionsforcomponentstorageLeadTimeDomestic:within1weekdelivery International:1weekIntendedUseResearchUseOnlyAdditionalInformationIntendedUse:TheFibronectintestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringFibronectininbiologicalsamplesofrat.::Reagents:1.DILUENTCONCENTRATE(RunningBuffer)Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.2.WASHSOLUTIONCONCENTRATEOnebottlecontaining50mlofa20Xconcentratedwashsolution.3.ENZYME-ANTIBODYCONJUGATE100XOnevialcontaining150ulofaffinitypurifiedanti-RatFibronectinantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer.4.CHROMOGEN-SUBSTRATESOLUTIONOnevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.5.STOPSOLUTIONOnevialcontaining12ml0.3Msulfuricacid.WARNING:Avoidcontactwithskin.6.ANTI-RATFIBRONECTINELISAMICROPLATETwelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-RatFibronectin.7.RATFIBRONECTINCALIBRATOROnevialcontainingalyophilizedRatFibronectincalibrator.

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