首页 > GenWay > GenWay/Rubella Virus IgM μ-capture ELISA/GWB-1D2E67/1x96 Assays

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GenWay/Rubella Virus IgM μ-capture ELISA/GWB-1D2E67/1x96 Assays

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GWB-1D2E67

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Description:PleaseclickhereforMSDSPDFDatasheetProductName:RubellaVirusIgMμ-captureELISA#ofSamples:1x96AssaysIntendedUse:TheRubellaVirusIgMμ-capture-ELISAisintendedforthequalitativedeterminationofIgMclassantibodiesagainstRubellaVirusIgMinhumanserum.Introduction:RubellaisanenvelopedRNAvirusbelongingtothetogaviruses.Ithasasphericalshapemeasuringabout50-70nmindiameter.Thereappearstobeonlyoneantigenictype,andnocross-reactivitywithalphavirusesorothermembersofthetogavirusgrouphasbeenfound.Rubellavirusesarepathogensoftherespiratorytractandtransmittedmainlybydropletinfection.Rubellaisaworldwidecommoncontagiousdiseasewithmildconstitutionalsymptomsandageneralizedrush.Inchildhood,itisaninconsequentialillness,butwhenitoccursduringpregnancy,thereisasignificantriskofseveredamagetothefetus.Theriskofcongenitalrubelladependsprimarilyonthemonthofpregnancyinwhichinfectionisacquired:overall,app.16%ofinfantshavemajordefectsatbirthfollowingmaternalrubellainthefirst3monthsofpregnancy.Congenitalrubellainfectionmayleadtoasyndromewithsingleormultipleorganinvolvements,knownasembryopathiarubeolosa.Insomecasesinfectionisinapparentbutresultsinconsequentialdamagesaseyedefects,deafness,growthretardation,andothers.Naturallyacquiredimmunityusuallyislong-lasting,butreinfectionispossIBLeduetodecreasinglevelsofcirculatingantibodies.Forimmunizationavaccinecontaininglivevirusisused.InfectionmaybeidentifiedbyDetectionofvirusbyPCR(prenatal)Hemagglutinationinhibition(HAI),Haemolysis-in-geltest(HiG)DetectionofantibodiesbyEIA,ELISAMeasurementofantibodiesintheserumisimportantforthedeterminationoftheimmunestatus.Evenapreviousinfectionthoughratherovertmaynotyieldalong-lastingimmunity,butmayresultinanantibodytitertoolowtopreventreinfection.Especiallythescreeningofadolescentsandyoungwomenshouldbeamandatoryroutineinprenatalcare.Principlesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgM-classantibodiesagainstRubellaVirusisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.TheRubellaVirusIgMELISAisanIgMμ-captureELISA.Theplatesarecoatedwithanti-humanIgM.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidaselabelledRubellavirusantigenconjugateisadded.ThisconjugatebindstothecapturedRubella-specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedwithTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofRubella-specificIgMantibodiesinthespecimen.Sulfuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasSEROlogicaldata.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesReefSe.,FreyTk.,TheallK.,AabernathyE.,BurnettCl.,IcenoglJ.,McCauleyMm.,WhartonM.Thechangingepidemiologyofrubellainthe1990s:onthevergeofeliminationandnewchallengesforcontrolandprevention.JAMA.2002Jan23-30;287(4):464-72.MezzasomaLBacarese-HamiltonT.,DiChristinaM.,RossiR.BistoniF.,CrisantiA.Antigenmicroarraysforserodiagnosisofinfectiousdiseases.ClinChem.2002Jan;48(1):121-30.CooperLz.Currentlessonsfrom20thcenturyserosurveillancedataonrubella.ClinInfectDis.2001Oct15;33(8):1287.SignoreC.Rubella.Prim.CareUpdateObGyns.2001Jul;8(4):133-137.WeberB.Currentdevelopmentsinthelaboratorydiagnosisofrubella.BullSocSciMedGrandDucheLuxemb.1997;134(2):31-41.ThomasHI,BarrettE,HesketLM,WynneAandMorgan-CapnerP.SimultaneousIgMreactivitybyEIAagainstmorethanonevirusinmeasles,parvovirusB19andrubellainfection;JournalofclinicalVirology1999,14:107118.PinskyNA,HuddelstonJM,JacobsonRM,WollanPCandPolandGA.Effectofmultiplefreeze-thawcyclesondetectionofmeasles,mumps,andrubellavirusantibodies;Clin.Diagn.Lab.Immunol.2003,10(1):19-21.TipplesGA,Hamkar,R,Mohktari-AzadT,GrayM,BallJ,HeadCandRatnamS;EvaluationofrubellaIgMenzymeimmunoassays.J.Clin.Virol.2004,30:233-238.AdditionalInformation:NameRubellaVirusIgMμ-captureELISARelatedProductNamesRubellaVirusIgMμ-captureELISAStorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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