Description:1.IntendedUse TheGenWayAdenovirusIgMAntibodyELISATestKithasbeendesignedforthethedetection andthequantitativedeterminationofspecificIgMantibodiesagainstAdenovirusinserumand plasma.FurtherapplicationsinotherbodyfluidsarepossIBLeandcanberequestedfromthe TechnicalServiceofGenWay. Thisassayisintendedforresearchuseonly. 2.GeneralInformation Theadenovirusisanubiquitouspathogenofhumansandanimals.Adenovirusesarecharacterized bylocationinsidethecellnucleus,commoncomplement-fixingantigensandmarkedstABIlityto environmentaleffects.Adenovirusesareendemicinallpopulationsthroughouttheyear.The infectionisspreadboththroughtheaerial-dropletrouteandtheroutescharacteristicforintestinal infections.Theincubationperiodisbetweenfiveandsevendays.Adenovirusesmainlyinfest respiratoryandintestinalmucosa,butalsothecornea.Theyareaccumulatedintheepithelialcells andregionallymphnodes.Adenovirusescausethewidestvarietyofillnessesoftheknown respiratoryviruses.Theadenovirusinfectionis themostfrequentlycausedviraldiseaseofthe respiratorytractamongpreschoolchildren(types1-5and7).Acutediseasesoftheupper respiratorytractoccurpredominantly.Pneumoniaisthemostsevereformofadenoviralinfection occurringmostlyininfantsbelowtheageofone.Adenovirusesalsocauseoutbreaksofswimmingpool-associatedpharyngoconjunctivalfeverinthesummerandepidemicsofkerato-conjunctivitis ofbothchildrenandadults.Theintestinalformofadenoviralinfectionoccursmostlyinchildren belowtheageofone.Anacuteadenoviralinfectioncanbedetectedbyvirusisolationand/or SEROlogy.Theserologictestsareparticularlyimportantbecausetheydocumentactualinfectionin thepatientandcanbeappliedtolarge-scaleepidemiologicinvestigations.TheCFandELISAtests measurepredominantlytheantibodiesdirected againstthegroup-specificdeterminantsonthe hexoncomponent.Therecommendedtestsfor measuringtype-specificantibodiesare hemagglutinininhibitionandserumneutralization. Thetype-specificantigenicdeterminantsof adenovirusesarelocatedatthefibersonthecapsid.Becauseoftheubiquityoftheadenovirusesand numerouscross-reactionsbetweenrelatedserotypes,seroconversioninvolvingafourfoldorgreater riseinantibodyinfectionisnecessarytodocumentinfection.IgGisthepredominantantibodyclass measuredintheserologictests. 3.PrincipleoftheTest TheGenWayAdenovirusIgMantibodytestkit isbasedontheprincipleoftheenzyme immunoassay(EIA).Adenovirusantigenisboundonthesurfaceofthemicrotiterstrips.Diluted patientserumorready-to-usestandardsarePipettedintothewellsofthemicrotiterplate.Abinding betweentheIgMantibodiesoftheserumandtheimmobilizedAdenovirusantigentakesplace. Afteraonehourincubationatroomtemperature,theplateisrinsedwithdilutedwashsolution,in ordertoremoveunboundmaterial.Thenready-to-useanti-human-IgMperoxidaseconjugateis addedandincubatedfor30minutes.Afterafurtherwashingstep,thesubstrate(TMB)solutionis pipettedandincubatedfor20minutes,inducingthedevelopmentofabluedyeinthewells.The colordevelopmentisterminatedbytheadditionofastopsolution,whichchangesthecolorfrom bluetoyellow.Theresultingdyeismeasuredspectrophotometricallyatthewavelengthof450nm. TheconcentrationoftheIgMantibodiesisdirectlyproportionaltotheintensityofthecolor. AdditionalInformation:NameAdenovirusIgMELISARelatedProductNamesAdenovirusIgMELISAApplicationsEnzymeimmunoassaybasedonmicrotiterplateforthedetectionandquantitativedeterminationofhumanIgMantibodiesagainstAdenovirusinserumandplasmaIntendedUseResearchUseOnly