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GenWay/TOTAL ESTRIOL/GWB-1FCEC3/1x96 Assays

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GWB-1FCEC3

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Description:ProductName:TotalEstriol#ofSamples:1x96AssaysIntendedUse:CompetitiveimmunoenzymaticcolorimetricmethodforquantitativedeterminationofEstriolinserumorplasma.Introduction:Estriolisafemalesexsteroidhormoneandoneofthethreemajornaturallyoccurringestrogens,theothersbeingestrADIolandestrone.Estriolisproducedalmostexclusivelyduringpregnancyandisthemajorestrogenproducedinthenormalhumanfetus.DuringpregnancytheproductionofEstrioldependsonanintactmaternal-placental-fetalunit.Fetal-placentalproductionofEstriolleadstoaprogressiveriseinmaternalcirculatinglevelsreachingalate-gestationalpeakseveralordersofmagnitudegreaterthannon-pregnantlevels.Innon-pregnantcircumstances,Estriolisderivedalmostexclusivelyfrom17β-Estradiol.EstriolexistsinBIOLOGicalmatrixinfree(ca.9%)andconjugatedform(91%).Conjugationwithglucuronicorsulphuricacidofestrioloccursintheliver,whichaidesinsteroidsolubility,thereforeallowingrapideliminationviathekidney.Principlesoftheassay:Microtiterstripwellsareprecoatedwithanti-Estriolantibodies(solid-phase).EstriolinthesamplecompeteswithaddedhorseradishperoxidaselabelledEstriol(enzyme-labelledantigen)forantibodybinding.Afterincubationabound/freeseparationisperformedbysolid-phasewashing.Theimmunecomplexformedbyenzyme-labelledantigenisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisinverselyproportionaltotheamountofEstriolinthesample.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorptionat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.ReferencesFisher-Rasmussen,W.,Gabrielsen,M.V.andWisborg(1981)ActaObstet.GynecolScand60,417-420.Truran,P.L.,Read,G.F.andWalker,S.(1982)Clin.Chem.28(12),2393.Vining,R.F.,McGinley,R.andRiceB.(1983)J.Clin.Endoc.Metab.56,454.Bagger,P.V.,Jacobsson,K.andGullberg,B.(1981)ActaObstetGynecolScand60,187.Osterman,T.M.,Juntunen,K.O.andGothoni,G.D.(1979)Clin.Chem.25(5),716.Wisdom,G.B.(1976)ClinChem.22(8),1243-1255.AdditionalInformation:NameTOTALESTRIOLRelatedProductNamesTotalEstriolMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark.IntendedUseResearchUseOnly

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