Description:1.IntendedUse TheGenWayInfluenzaBIgAAntibodyELISATestKithasbeendesignedforthethedetection andthequantitativedeterminationofspecificIgAantibodiesagainstInfluenzaBinserumand plasma.FurtherapplicationsinotherbodyfluidsarepossIBLeandcanberequestedfromthe TechnicalServiceofGenWay. Thisassayisintendedforresearchuseonly. 2.GeneralInformation Theinfluenzainfectionisanacutefeverishvirusinfection,whichprincipallyleadstoanillnessof therespiratorytractandappearsasanepidemicorpandemic.Theinfectionmostlyresultsfroma dropletinfection.Thevirusspreadsfromthemucousmembraneoftheupperrespiratorytothe wholebronchialtract.Therethevirusanditstoxincanleadtoaseriousinflammationofthe bronchialmucosaandadamageofthevessels. Afteranincubationtimeof1to3daysthe symptomsappearsuddenly:Followedbyafast increaseoftemperature,oftenaccompaniedby shivering,thecatarrhalleADIngsymptomappears,whichcontributetothecoursebesidepainfuldry cough,tracheitis,laryngitisandfrequentlyarhinitisandconjunctivitis. TheInfluenzavirusesformavirusgroupwith principallysimilarmorphological,chemicaland BIOLOGicalfeatures.ThetypesA,BandCweredefined,fromwhichmanyothervariantsareknown. Thedistinctionofthetypeswillbepossiblebythedifferentantigenicityoftheirnucleoproteins, whicharecoatedbyamatrixproteinwithtype-specificantigenicity,too.However,bothinternal antigensareoflessimportancefortheimmunity.Theessentialantigensarethehemagglutininand theneuraminidase.Botharesurfaceantigensandsubjecttoapermanentchangeoftheir antigenicity,whichiscalleddriftorshift.TheappearenceofpermanentnewInfluenzaepidemics andpandemicsareparticularlyfacilitatedbyanantigenvariABIlity,becausethenewdriftorshift variantsinfectapopulationwhichisonlypartlyimmuneorinanextremecasecompletely susceptibletothedisease. ThedeterminationoftheInfluenzatype(A,B,andC)givesboththeclinicianandepidemiologist importantindicationsforfurtheractions.ThusInfluenzaBoftenleadstoaseriouscourseandan epidemicspreadofthevirus.Similarly,duringanInfluenzaAepidemic,theepidemiological importanceandderivedmeasuresfortheprotectionoftheindividualandpopulationprimarilystand intheforegroundtogetherwiththeseverityofthesymptoms. 3.PrincipleoftheTest TheGenWayInfluenzaBIgAantibodytestkit isbasedontheprincipleoftheenzyme immunoassay(EIA).InfluenzaBantigenisboundonthesurfaceofthemicrotiterstrips.Diluted sampleserumorready-to-usestandardsarePipettedintothewellsofthemicrotiterplate.Abinding betweentheIgAantibodiesoftheserumandtheimmobilizedInfluenzaBantigentakesplace.After aonehourincubationatroomtemperature,theplateisrinsedwithdilutedwashsolution,inorderto removeunboundmaterial.Thenready-to-useanti-human-IgAperoxidaseconjugateisaddedand incubatedfor30minutes.Afterafurtherwashingstep,thesubstrate(TMB)solutionispipettedand incubatedfor20minutes,inducingthedevelopmentofabluedyeinthewells.Thecolor developmentisterminatedbytheadditionofastopsolution,whichchangesthecolorfromblueto yellow.Theresultingdyeismeasuredspectrophotometricallyatthewavelengthof450nm.The concentrationoftheIgAantibodiesisdirectlyproportionaltotheintensityofthecolor.AdditionalInformation:NameInfluenzaBIgAELISARelatedProductNamesInfluenzaBIgAELISAIntendedUseResearchUseOnly