首页 > GenWay > GenWay/Rat Resistin ELISA Kit/GWB-22CF20/ELISA_Kits

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GenWay/Rat Resistin ELISA Kit/GWB-22CF20/ELISA_Kits

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GWB-22CF20

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GenWay

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4000-520-616

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Description:ProductName:RatResistinELISA#ofSamples:1X96AssaysIntendedUse:TheRatResistinELISAisabiotinlabelledantibodybasedsandwichenzymeimmunoassayforthequantitativemeasurementofratresistininserumandtissueculturemedium.Itisintendedforinvitroandresearchuseonly.Principleofthetest:InGenWay"sRatResistinELISA,calibratorsorsamplesareincubatedwitharabbitpolyclonalanti-ratresistinantibodycoatedinmicrotiterwells.Afterone-hourincubationandawashing,biotin-labelledrabbitpolyclonalantiratresistinantibodyisaddedandincubatedwithcapturedresistin.Afterathoroughwash,streptavidin-horserADIshperoxidaseconjugateisadded.Afteronehourincubationandthelastwashingstep,theremainingconjugateisallowedtoreactwiththesubstrateH2O2-tetramethylbenzidine.Thereactionisstoppedbyadditionofacidicsolutionandabsorbanceoftheresultingyellowproductismeasuredat450nm.Theabsorbanceisproportionaltotheconcentrationofresistin.Astandardcurveisconstructedbyplottingabsorbancevaluesversusresistinconcentrationsofcalibrators,andconcentrationsofunknownsamplesaredeterminedusingthisstandardcurve.StorageandStABIlity:Storethekitat2-8C.Undertheseconditions,thekitisstableuntiltheexpirationdate(seelabelonthebox).LimitationoftheTest:Resultsexceedingresistinlevelof10ng/mlshouldberepeatedwithdilutedsamples.Dilutionfactorsneedtobetakenintoconsiderationincalculatingtheresistinconcentration.SensitivityandSpecificity:TheantibodiesinRatResistinELISAkitarehighlyspecificforratresistinwithnodetectablecrossreactivitiestohumanandmouserecombinantresistinat50ng/ml.Nosignalwasfoundtothefollowingsera:human,mouse,rabbit,horse,goat,pig,hamster,chicken,monkey,sheepandbovine.ProductName:RatResistinELISAFunction:Hormonethatseemstosuppressinsulinabilitytostimulateglucoseuptakeintoadiposecells.Potentiallylinksobesitytodiabetes.Subunit:Homodimer;disulfide-linked(Bysimilarity).SubcellularLocation:Secreted.TissueSpecificity:Expressedonlyinfattytissues.Similarity:Belongstotheresistin/FIZZfamily.AdditionalInformation:NameRatResistinELISAKitRelatedProductNamesRatResistinELISAKitELISAKit;Cysteine-richsecretedproteinFIZZ3;Adiposetissue-specificsecretoryfactor;ADSF;C/EBP-epsilon-regulatedmyeloid-specificsecretedcysteine-richprotein;Cysteine-richsecretedproteinA12-alpha-like2FIZZ3;HXCP1;NCBIAccNumberNP_065148.1MolecularWeight11419SwissProtNumberQ9HD89IntendedUseResearchUseOnly

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