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GenWay/Influenza Virus B IgG/GWB-27F665/1x96 Assays

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GWB-27F665

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Description:PleaseclickhereforMSDSPDFDatasheetProductName:InfluenzaVirusBIgG-ELISA#ofSamples:1x96AssaysIntendedUse:TheInfluenzaVirusBIgG-ELISAisintendedforthequalitativedeterminationofIgGclassantibodiestoInfluenzaVirusBinhumanserumorplasma(citrate).Introduction:InfluenzaareRNAvirusesofthefamilyOrthomyxoviridae.Influenzavirusesaredividedintothreetypes,designatedA,B,andCwhicharedifferentiatedbythespecificityofasolubleantigenassociatedwiththeinternalribonucleoproteincomponentofthevirion.Thevirionsarespericalparticlesof80-120nmindiameterconsistingoftheribonucleoproteincomponentandenvelopedbymatrixproteinandalipidbilayerwhichcontainstwospikelinestructures:viralhemagglutinin(H)andviralneuraminidase(N).Influenzavirusesarerespiratorytractpathogenswhicharetransmittedbydirectcontact,large-dropletinfection,orbycontaminatedsurfaces.InfluenzatypesAandBareresponsIBLeforepidemicsofrespiratoryillnessthatoccuralmosteverywinterandareoftenassociatedwithincreasedratesofhospitalizationanddeath.TypeCinfectionsusuallycauseeitheraverymildrespiratoryillnessornosymptomsatall;itdoesnotcauseepidemicsanddoesnothavetheseverepublichealthimpactthatinfluenzatypesAandBdo.PandemicsofinfluenzavirustypeAinfectionshaveoccuredat10to20yearintervalssince1890.ApparentlythisresultsfromalterationsinthecompositionoftheHandNantigens(antigenicdriftandantigenicshift).CurrentlytwosubtypesofinfluenzaAarecirculatingworldwide.Normallyinfluenzaisaself-limitingdiseaselastingfor3to7days,butsomepeopledevelopseriousandpotentiallylife-threateningmedicalcomplications,suchaspneumonia,particularlyinchildren,elderlypeopleandothervulnerablegroups.Thepresenceofvirusresp.infectionmaybeidentifiedbyMicroscopy:CPE,IFPCRSEROlogy:Hemadsorption,Hemagglutination-Inhibition,DetectionofantibodyproductionbyELISAPrinciplesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgG-classantibodiestoInfluenzaVirusBisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.MicrotiterstripwellsareprecoatedwithInfluenzaVirusBantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedInfluenzaVirusBspecificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofInfluenzaVirusBspecificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.ReferencesKlenk,mH.D.,R.Rott:ThemolecularBIOLOGyofinfluenzaviruspathogenicity.Adv.Virus.Res.34(1988)247-281Palese,P.,D.K.Kingsburg(Hrsg.):Geneticsofinfluenzaviruses.Springer,Wien,NewYork(1983).Webster,R.G.,W.J.Bean,O.T.Gormanetal.:evolutionandecologyofinfluenzaAviruses.Microbiol.Rev.56(1992)152-179Assaad,F.,G.Torrigiani:immunologicalaspectsofthzepreventionofviraldiseases.Bull.WorldHealthOrgan65(1987)1-10Couch,R.B.,J.A.Kasal,W.P.Glezenetal.:Influenza:itscontrolinpersonsandpopulation.J.Infect.Dis.153(1986)431-440AdditionalInformation:NameInfluenzaVirusBIgGRelatedProductNamesInfluenzaVirusBIgG-ELISAMolecularWeight0.5StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.IntendedUseResearchUseOnly

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