首页 > GenWay > GenWay/Thyroid Stimulating Hormone ELISA Kit/GWB-29CB3E/96 TESTS

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GenWay/Thyroid Stimulating Hormone ELISA Kit/GWB-29CB3E/96 TESTS

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GWB-29CB3E

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GenWay

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Description:ProductName:ThyroidStimulatingHormoneCategory:ThyroidFunctionSampleType:SerumandplasmaSensitivity:0.05IU/mLDescription:ImmunoenzymetricassayfortheinvitroquantitativemeasurementofhumanThyroidStimulatingHormone(TSH)inserumandplasmaIntendedUse:ImmunoenzymetricassayfortheinvitroquantitativemeasurementofhumanThyroidStimulatingHormone(TSH)inserumandplasma.Background:BIOLOGicalactivitiesThyrotropecellsoftheanteriorpituitarysynthesizeandsecretehumanthyroidstimulatinghormone(TSH),aglycoproteinofmolecularweight28,000Da,comprisingtwosubunits?:-TSHisverysimilartoa?subunitofLH,FSHandhCG,?-TSHdiffersfromotherhormonesubunitsanddefinestheimmunologicalspecificity.TSHregulatesthesynthesisandreleaseofthyroidhormones:thyroxin(T4)andtriiodothyronine(T3).TSHsecretionisstimulatedbyahypothalamicpeptide,TRH(TSHreleasinghormone);anegativefeedbackonTSHsecretionisexertedbyT3andT4.Principlesofthemethod:TheGenWayTSH-EASIAisasolidphaseEnzymeAmplifiedSensitivityImmunoassayperformedonmicrotiterplates.Calibratorsandsamplesreactwiththecapturemonoclonalantibody(MAb1)coatedonmicrotiterwellandwithamonoclonalantibody(MAb2)labelledwithhorserADIshperoxidase(HRP).Afteranincubationperiodallowingtheformationofasandwich:coatedMAb1-humanTSH-MAb2-HRP,themicrotiterplateiswashedtoremoveunboundenzymelabelledantibody.Boundenzyme-labelledantibodyismeasuredthroughachromogenicreaction.Chromogenicsolution(TMB-H2O2)isaddedandincubated.ThereactionisstoppedwiththeadditionofStopSolutionandthemicrotiterplateisthenreadattheappropriatewavelength.Theamountofsubstrateturnoverisdeterminedcolourimetricallybymeasuringtheabsorbance,whichisproportionaltotheTSHconcentration.AcalibrationcurveisplottedandTSHconcentrationinsamplesisdeterminedbyinterpolationfromthecalibrationcurve.TheuseoftheEASIAreader(linearityupto3ODunits)andasophisticateddatareductionmethod(polychromaticdatareduction)resultinahighsensitivityinthelowrangeandinanextendedcalibrationrange.StorageandStABIlity:Beforeopeningorreconstitution,allkitcomponentsarestableuntiltheexpirydate,indicatedontheviallabel,ifkeptat2to8C.Unusedstripsmustbestored,at2-8C,inasealedbagcontainingadesiccantuntilexpirationdate.Afterreconstitution,calibratorsandcontrolsarestablefor1weekat2to8C.Forlongerstorageperiods,aliquotsshouldbemadeandkeptat-20C.Avoidsuccessivefreezethawcycles.TheconcentratedWashSolutionisstableatroomtemperatureuntilexpirationdate.FreshlypreparedWorkingWashsolutionshouldbeusedonthesameday.Afteritsfirstuse,theconjugateisstableuntilexpirydate,ifkeptintheoriginalwell-closedvialat2to8C.Thefreshlypreparedrevelationsolutionisstable,beforeuse,formaximum15minutesatroomtemperatureandmustbediscardedafterwards.Alterationsinphysicalappearanceofkitreagentsmayindicateinstabilityordeterioration.ProductName:ThyroidStimulatingHormoneSubunit:Heterodimerofacommonalphachainandauniquebetachainwhichconfersbiologicalspecificitytothyrotropin,lutropin,follitropinandgonadotropin.SubcellularLocation:Secreted.Similarity:Belongstotheglycoproteinhormonessubunitalphafamily.AdditionalInformation:NameThyroidStimulatingHormoneELISAKitRelatedProductNamesThyroidStimulatingHormoneELISAKit;Anteriorpituitaryglycoproteinhormonescommonsubunitalpha;Follitropinalphachain;Follicle-stimulatinghormonealphachain;FSH-alpha;Lutropinalphachain;Luteinizinghormonealphachain;LSH-alpha;ThyrotropNCBIAccNumberNP_000726.1MolecularWeight13075SwissProtNumberP01215IntendedUseResearchUseOnly

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