Description:ShortDescription:Transferrin(TX)isametal-combiningproteinthatreversIBLybindstoacid-solubleironinplasma.Itfunctionstotransportirontothebonemarrowandtotissuestorageorganssuchastheliver.Transferrinalsoparticipateintheregulationandcontrolofironabsorptionandprotectsagainstironintoxication.Likehaptoglobin,thecarrierofhemoglobin,transferrinissynthesizedintheliver,butunlikehaptoglobintransferrinisreturnedtothecirculationafterunloADIngitsironinthereticuloendothelialsystem.ThisELISAkitcanbeusedtomeasuretransferrininserum,tissueextractsandotherBIOLOGicalfluids.AdditionalInformation:NameRatTransferrinELISAKitRelatedProductNamesRatTransferrinELISAKit;Transferrin;Siderophilin;Beta-1-metal-bindingglobulinRatTransferrintrfeGeneSymbolTFGeneFullnametransferrinGeneid24825AliasSymbolsSEROtransferrin,transferrin,Beta-1metal-bindingglobulin,TF,SiderophilinProteinNameSerotransferrinDescriptionofTargetTransferrinsareironbindingtransportproteinswhichcanbindtwoFe3+ionsinassociationwiththebindingofananion,usuallybicarbonate.ItisresponsibleforthetransportofironfromsitesofabsorptionandhemedegradationtothoseofstorageaSwissprotIDP12346SpeciesReactivityRatAssayInfoAssayMethodology:QuantitativeSandwichELISASensitivity1.153ng/mlKitRange6.25ng/ml-400ng/mlHomologyRatNCBIAccNumberNP_001054.1StandardRangeReferToManualSampleTypeSerum,plasmaandotherbiologicalfluidsProductFormatInSolutionSpecimen/VolumesReferToManualSwissProtNumberP02787ApplicationsELISAReconstitutionandStorage1.DILUENTThe5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.2.WASHSOLUTIONThe20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.3.ENZYME-ANTIBODYCONJUGATEUndilutedhorseradishperoxidaseanti-TXconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.4.CHROMOGEN-SUBSTRATESOLUTIONTheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.5.STOPSOLUTIONTheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.6.ANTI-RATTRANSFERRINELISAMICROPLATEAnti-RatTXcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.7.RATTRANSFERRINCALIBRATORThelyophilizedRatTransferrincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.ReactivityRatKitSize96wellKitPrincipleGenWayBiotechTransferrinELISAKit(Rat)(GWB-2C5ED4)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforSerotransferrinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Serotransferrin-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleSerotransferrincapturedinthewell.KitComponentComponentAmountAnti-RatTransferrin_x000D_Microplate96Wells(12x8WellStrips)20XDiluentConcentrate50mL20XWashSolution50mL100XEnzyme-AntibodyConjugate150uLChromogen-SubstrateSolution12mLStopSolution12mLRatTransferrin_x000D_Calibrator1KitDuration40MinutesStorageRefertokitinstructionsforcomponentstorageLeadTimeDomestic:within1weekdelivery International:1weekIntendedUseResearchUseOnlyAdditionalInformationIntendedUse:TheTransferrintestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringTransferrininbiologicalsamplesofrats.::Reagents:1.DILUENTCONCENTRATE(RunningBuffer)Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.2.WASHSOLUTIONCONCENTRATEOnebottlecontaining50mlofa20Xconcentratedwashsolution.3.ENZYME-ANTIBODYCONJUGATE100XOnevialcontaining150ulofaffinitypurifiedanti-RatTransferrinantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer.4.CHROMOGEN-SUBSTRATESOLUTIONOnevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.5.STOPSOLUTIONOnevialcontaining12ml0.3Msulfuricacid.WARNING:Avoidcontactwithskin.6.ANTI-RATTRANSFERRINELISAMICROPLATETwelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-RatTX.7.RATTRANSFERRINCALIBRATOROnevialcontainingalyophilizedRatTransferrincalibrator.